Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase RHF1A (RHF1A)

Code CSB-YP700194DOA
MSDS
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Source Yeast
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Code CSB-EP700194DOA
MSDS
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Source E.coli
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Code CSB-EP700194DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP700194DOA
MSDS
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Source Baculovirus
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Code CSB-MP700194DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RHF1A
Uniprot No.
Alternative Names
RHF1A; At4g14220; dl3150w; FCAALL.146; E3 ubiquitin-protein ligase RHF1A; EC 2.3.2.27; RING-H2 finger F1a; RING-H2 zinc finger protein RHF1a; RING-type E3 ubiquitin transferase RHF1A
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-371
Target Protein Sequence
MSNFTYTSAF NLSDNSPFNP AIGSSSSSSS ALVVASDDDN NTDDACSICL EPFTLQDPST VTSCKHEYHL QCIIEWSQRS KECPICWQLF VLRDPASQEL LAAVEKERLL KTRNISSSSP ISIHHSHDDF HSEEEESQFS SFDEQFLRHL TEAAHRRCLL RRRDGQISSS LVSSSDPTTI HPTDLVNLYR LSAISHVEHQ NSNPCPSPGS MTPSPVSGHS SIPADSNNGS RISPGPSPSR SSQSPKSPEA SSLPEAIKSK LAAASAKYKE SISKSKQGLK EKLLARNNSV KELSKGVQRE MNAGIAGVAR MIERMDFSSK RFGGSAHVST STATASGFNF SFKGKRVEAN SKSNNNGDKT EPQKLQGGET C
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
E3 ubiquitin-protein ligase involved in the positive regulation of the gametogenesis progression. Mediates the proteasomal degradation of KRP6, a cyclin-dependent kinase inhibitor which accumulates during meiosis and blocks the progression of subsequent mitoses during gametophyte development. Functions in association with RHF2A. Possesses E3 ubiquitin-protein ligase activity when associated with the E2 enzyme UBC8 in vitro.
Tissue Specificity
Expressed in stems, flowers, green siliques, cauline leaves, seeds and roots.
Database Links

KEGG: ath:AT4G14220

STRING: 3702.AT4G14220.1

UniGene: At.24002

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