Recombinant Arabidopsis thaliana ETHYLENE INSENSITIVE 3-like 1 protein (EIL1), partial

Code CSB-YP886721DOA
MSDS
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Source Yeast
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Code CSB-EP886721DOA
MSDS
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Source E.coli
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Code CSB-EP886721DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP886721DOA
MSDS
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Source Baculovirus
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Code CSB-MP886721DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
EIL1
Uniprot No.
Alternative Names
EIL1; At2g27050; T20P8.10; ETHYLENE INSENSITIVE 3-like 1 protein
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probable transcription factor acting as a positive regulator in the ethylene response pathway. Could bind the primary ethylene response element present in the ETHYLENE-RESPONSE-FACTOR1 promoter.
Gene References into Functions
  1. EIN3/EIL1 and RHD6/RSL1 coordinately enhance root hair initiation by selectively regulating a subset of core root hair genes. PMID: 29233944
  2. these data suggest the detailed mechanism of DNA recognition by EIN3 and provide an in-depth view at molecular level for the transcriptional regulation mediated by EIN3. PMID: 26352699
  3. High salinity induces EIN3 and EIL1 protein accumulation and regulates EIL1 dependent gene network. PMID: 25330213
  4. EIN3/EIL1 physically interact with FIT, are required for full FIT accumulation, and contribute to full FIT downstream target gene expression. PMID: 21586684
  5. Photo-oxidative stress may increase upon iron deficiency, which is prevented by expression of EIN3/EIL1. PMID: 22212120
  6. identify EIN3/EIL1 as a key integration node whose activation requires both jasmonate and ethylene signaling as a common mechanism to integrate diverse signaling pathways in the regulation of plant development and defense PMID: 21737749
  7. our studies demonstrate that ethylene similarly regulates EIN3 and EIL1, the two master transcription factors coordinating myriad ethylene responses, and clarify that EIN2 but not MKK9 is required for ethylene-induced EIN3/EIL1 stabilization PMID: 20647342
  8. Transcription factor EIL1 is required for the second phase but not the first phase of growth inhibition by ethylene in Arabidopsis seedling hypocotyls. PMID: 15466219
  9. Light induced regulation of gene expression involves stabilization of EIL1 protein. PMID: 17011517
  10. Data provide evidence that EIN3/EIL1 directly target SID2 to downregulate PAMP defenses. PMID: 19717619

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Subcellular Location
Nucleus.
Protein Families
EIN3 family
Database Links

KEGG: ath:AT2G27050

STRING: 3702.AT2G27050.1

UniGene: At.10809

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