Recombinant Arabidopsis thaliana Elongator complex protein 4 (ELP4)

Code CSB-YP874907DOA
MSDS
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Source Yeast
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Code CSB-EP874907DOA
MSDS
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Source E.coli
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Code CSB-EP874907DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP874907DOA
MSDS
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Source Baculovirus
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Code CSB-MP874907DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ELP4
Uniprot No.
Alternative Names
ELP4; ELO1; At3g11220; F11B9.14Elongator complex protein 4; AtELP4; Elongator component 4; Protein ELONGATA 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-355
Target Protein Sequence
MAAPNVRSSS SFSRNISVVS SPQIPGLKSG PNGTAFISSG IRDLDRILGG GYPLGSLVMV MEDPEAPHHM DLLRTYMSQG LVNNQPLLYA SPSKDPKGFL GTLPHPASSK EDKPTAPDPD QGESLRIAWQ YRKYLENQKN AIDDYSNDFD MRKPLERQFL SGRPIDCVSL LDSSDLSIAQ DHCATFLSKF PRNSSNIASI GRIAIQSFCS PLCEYSEKES DMLSFIRLLK SMLMVSNAVA IVTFPPSLLS PSSSKRLQHM ADTLLSIKAI PDGDKELEKL LTGYKDINGF LNIHKVARIN TQVPVILEAK TFSMSLKKRR FLALECLNQA PVDGSSGTSY GTSGSCSSKS GALDF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of the RNA polymerase II elongator complex, a multiprotein complex associated with the RNA polymerase II (Pol II) holoenzyme, and which is involved in transcriptional elongation. The elongator complex catalyzes formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. Promotes organs development by modulating cell division rate. May regulate mechanisms producing carbon assimilates or importing sucrose. Involved in the repression of the abscisic acid (ABA) signaling pathway during seed germination and seedling growth. Required for auxin distribution or signaling. Involved in oxidative stress signaling. Prevents anthocyanins accumulation.
Gene References into Functions
  1. show that the Elongator (elo1, elo2, and elo3) complex was purified from plant cell cultures as a six-component complex. PMID: 20080602
  2. Electron microscopy of an elo mutant showed a hypotonic vacuole, alterations in the size of grana and starch grains in the chloroplasts, and the massive presence of Golgi vesicles in the cytoplasm. PMID: 17565971
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
ELP4 family
Tissue Specificity
Expressed in meristematic tissues of roots, leaves, stems, seedlings, cotyledons, flowers, siliques, guard cells, floral buds, and shoot apices.
Database Links

KEGG: ath:AT3G11220

UniGene: At.27198

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