Recombinant Arabidopsis thaliana Endoglucanase 25 (KOR), partial

Code CSB-YP653629DOA
MSDS
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Source Yeast
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Code CSB-EP653629DOA
MSDS
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Source E.coli
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Code CSB-EP653629DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP653629DOA
MSDS
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Source Baculovirus
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Code CSB-MP653629DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KOR
Uniprot No.
Alternative Names
KOR; DEC; KOR1; RSW2; At5g49720; K2I5.8; Endoglucanase 25; EC 3.2.1.4; Cellulase homolog OR16pep; Endo-1,4-beta glucanase 25; Protein KORRIGAN; Protein RADIALLY SWOLLEN 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for cellulose microfibrils formation. Involved in cell wall assembly during cell elongation and cell plate maturation in cytokinesis. Required for secondary cell wall formation in the developing xylem. May cycle through different intracellular compartments, including plasma membrane.
Gene References into Functions
  1. N-glycosylation of the cellulase domain important for targeting to and retention within the trans-Golgi network PMID: 26039485
  2. These results suggest that one or several additional factor(s) carrying complex N-glycans cooperate(s) with KOR1 in trans to grant proper targeting/functioning in plant cells. PMID: 25238750
  3. KOR1 transmembrane domain has shown to be required for the interaction between KOR1 and the different CESAs, as well as for higher oligomer formation of KOR1 PMID: 25383767
  4. The jasmonic acid (JA) pathway was activated more in kor1-1 plants with an increase in the JA-biosynthesis gene LOX3 and a greater accumulation of JA. Upon infection the accumulation of JA and JA-isoleucine (JA-Ile) was higher than in wild-type plants. PMID: 25108263
  5. data indicate that utilization of several N-glycosylation sites is important for KOR1 activity, whereas the structure of the attached N-glycans is not critical PMID: 23782689
  6. KOR1 is required for maintaining a correct meristematic pattern and organ growth as well as for a normal hormonal response. PMID: 19826767
  7. KORRIGAN has a role in microtubule cortical array organization. PMID: 18583534

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Subcellular Location
Cell membrane; Single-pass type II membrane protein. Note=Cell plate.
Protein Families
Glycosyl hydrolase 9 (cellulase E) family
Tissue Specificity
Highly expressed in roots and stems, at intermediate levels in leaves and flowers, and at lower levels in siliques. Expressed in xylem (at protein level).
Database Links

KEGG: ath:AT5G49720

STRING: 3702.AT5G49720.1

UniGene: At.21098

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