Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF073 (ERF073)

Code CSB-YP810177DOA
MSDS
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Source Yeast
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Code CSB-EP810177DOA
MSDS
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Source E.coli
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Code CSB-EP810177DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP810177DOA
MSDS
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Source Baculovirus
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Code CSB-MP810177DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ERF073
Uniprot No.
Alternative Names
ERF073; At1g72360; T10D10.17Ethylene-responsive transcription factor ERF073
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-211
Target Protein Sequence
MSQSFELYPW LGSASDGKKK QSSRYKGIRR RPWGRWAAEI RDPIKGVRVW LGTFNTAEEA ARAYDLEAKR IRGAKAKLNF PNESSGKRKA KAKTVQQVEE NHEADLDVAV VSSAPSSSCL DFLWEENNPD TLLIDTQWLE DIIMGDANKK HEPNDSEEAN NVDASLLSEE LLAFENQTEY FSQMPFTEGN CDSSTSLSSL FDGGNDMGLW S
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional activator involved in the hypoxic stress response. Acts as a transcriptional activator via the cis-acting element GCC box. Plays a role in low oxygen signaling and contributes to tolerance to anoxia stress by enhancing anaerobic gene expression and ethanolic fermentation. Invovlved in the modulation of ethylene responses under both normoxia and hypoxia.; Is mostly responsible for transactivation of hypoxia-responsive genes.; Involved in root development through the regulation of root meristem cell division. Does not act as a transcriptional activator via the cis-acting CRT/DRE element.
Gene References into Functions
  1. AtERF73/HRE1 plays a role in H2O2 production by affecting the hypoxia-induced expression of Rboh genes in hypoxia signal transduction. PMID: 27995331
  2. AtERF73/HRE1 is involved in modulating ethylene responses under both normoxia and hypoxia. PMID: 21398256
  3. controlled by 3 pathways during hypoxia: an ethylene-dependent pathway, an ethylene-independent/H 2O 2-dependent pathway, and an ethylene and H 2O 2-independent pathway PMID: 24698791
  4. HRE1alpha functions as a transcription activator in the nucleus, and plays an important role in root development through regulation of root meristem cell division. PMID: 24728113
  5. rotein localized in the nucleus and functioned as an in vivo transcription activator as confirmed in transient assays and experiments. PMID: 15980186

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Subcellular Location
Nucleus.; [Isoform 2]: Nucleus.
Protein Families
AP2/ERF transcription factor family, ERF subfamily
Tissue Specificity
Expressed in roots and at lower levels in shoots.; [Isoform 2]: Highly expressed in roots and leaf trichomes. Expressed in rosette leaves, stems, cauline leaves and flowers.
Database Links

KEGG: ath:AT1G72360

STRING: 3702.AT1G72360.2

UniGene: At.35111

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