Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF071 (ERF071)

Code CSB-YP522776DOA
MSDS
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Source Yeast
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Code CSB-EP522776DOA
MSDS
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Source E.coli
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Code CSB-EP522776DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP522776DOA
MSDS
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Source Baculovirus
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Code CSB-MP522776DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ERF071
Uniprot No.
Alternative Names
ERF071; At2g47520; T30B22.18; Ethylene-responsive transcription factor ERF071
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-171
Target Protein Sequence
MCGGAIISDF IWSKSESEPS QLGSVSSRKK RKPVSVSEER DGKRERKNLY RGIRQRPWGK WAAEIRDPSK GVRVWLGTFK TADEAARAYD VAAIKIRGRK AKLNFPNTQV EEEADTKPGG NQNELISENQ VESLSEDLMA LEDYMRFYQI PVADDQSATD IGNLWSYQDS N
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional activator that binds specifically to the cis-acting element GCC box 5'-AGCCGCC-3', or to the CRT/DRE element 5'-[AG]CCGAC-3'. Plays an important role in root development via root cell expansion regulation. Transcriptional activator involved in the hypoxic stress response. Plays a role in low oxygen signaling and contributes to tolerance to anoxia stress by enhancing anaerobic gene expression and ethanolic fermentation. Plays a role in the regulation of hypoxia-induced root slanting. Contributes to the promotion of adventitious root elongation under hypoxia, an adaptation response that strengthens the root system in upper soil layers where oxygen shortage may last for shorter time periods. Transcriptional activator involved in the osmotic stress response. May play a role in defense response against the fungal pathogen Fusarium graminearum downstream of ethylene signaling.
Gene References into Functions
  1. Overexpression of the ERFVII member HRE2 inhibits root bending, suggesting that primary root growth direction at hypoxic conditions is antagonistically regulated by hypoxia and hypoxia-activated ERFVIIs. PMID: 28698356
  2. AtERF71/HRE2 binds to both GCC box and DRE/CRT, transactivates expression of genes downstream via GCC box or DRE/CRT, and plays an important role in root development through regulation of root cell expansion. PMID: 25344007
  3. AtERF71/HRE2 might function as a transcription factor involved in the response to osmotic stress as well as hypoxia. PMID: 21946064
  4. HRE1 and HRE2 encode two nuclear-localized ethylene response factors specifically up-regulated under low oxygen conditions. PMID: 20113439
Subcellular Location
Nucleus.
Protein Families
AP2/ERF transcription factor family, ERF subfamily
Database Links

KEGG: ath:AT2G47520

STRING: 3702.AT2G47520.1

UniGene: At.37019

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