Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF114 (ERF114)

Code CSB-YP875447DOA
MSDS
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Source Yeast
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Code CSB-EP875447DOA
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Source E.coli
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Code CSB-EP875447DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP875447DOA
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Source Baculovirus
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Code CSB-MP875447DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ERF114
Uniprot No.
Alternative Names
ERF114; EBE; At5g61890; K22G18.1; Ethylene-responsive transcription factor ERF114; ERF bud enhancer
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-248
Target Protein Sequence
MYGKRPFGGD ESEEREEDEN LFPVFSARSQ HDMRVMVSAL TQVIGNQQSK SHDNISSIDD NYPSVYNPQD PNQQVAPTHQ DQGDLRRRHY RGVRQRPWGK WAAEIRDPKK AARVWLGTFE TAESAALAYD EAALKFKGSK AKLNFPERVQ LGSNSTYYSS NQIPQMEPQS IPNYNQYYHD ASSGDMLSFN LGGGYGSGTG YSMSHDNSTT TAATTSSSSG GSSRQQEEQD YARFWRFGDS SSSPHSGY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional regulator of cell proliferation and axillary bud outgrowth. Involved in maintaining the structure of the shoot apical meristem as well as plastochron and phyllotaxy. Activates several genes involved in cell cycle regulation and dormancy breaking, including CYCD3-3, DPA, and BARD1. Strongly down-regulates DRM1, DRMH1, MARD1 and several genes encoding different types of cell wall-remodeling proteins.
Gene References into Functions
  1. At5g61890 transcription factor is highly expressed in proliferating cells, and it's expression affects shoot architecture in Arabidopsis. PMID: 23616605
Subcellular Location
Nucleus.
Protein Families
AP2/ERF transcription factor family, ERF subfamily
Tissue Specificity
Strongly expressed in proliferating cells. Detected in root tips, stipules, shoot apex, floral tissues, young siliques and abscission zones.
Database Links

KEGG: ath:AT5G61890

STRING: 3702.AT5G61890.1

UniGene: At.29079

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