Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor RAP2-4 (RAP2-4)

Code CSB-YP810181DOA
MSDS
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Source Yeast
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Code CSB-EP810181DOA
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Source E.coli
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Code CSB-EP810181DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP810181DOA
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Source Baculovirus
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Code CSB-MP810181DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RAP2-4
Uniprot No.
Alternative Names
RAP2-4; ERF059; At1g78080; F28K19.29; T11I11.1; Ethylene-responsive transcription factor RAP2-4; Ethylene-responsive transcription factor ERF059; Protein RELATED TO APETALA2 4
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-334
Target Protein Sequence
MAAAMNLYTC SRSFQDSGGE LMDALVPFIK SVSDSPSSSS AASASAFLHP SAFSLPPLPG YYPDSTFLTQ PFSYGSDLQQ TGSLIGLNNL SSSQIHQIQS QIHHPLPPTH HNNNNSFSNL LSPKPLLMKQ SGVAGSCFAY GSGVPSKPTK LYRGVRQRHW GKWVAEIRLP RNRTRLWLGT FDTAEEAALA YDKAAYKLRG DFARLNFPNL RHNGSHIGGD FGEYKPLHSS VDAKLEAICK SMAETQKQDK STKSSKKREK KVSSPDLSEK VKAEENSVSI GGSPPVTEFE ESTAGSSPLS DLTFADPEEP PQWNETFSLE KYPSYEIDWD SILA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Probably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways.
Gene References into Functions
  1. the WIND1-mediated signaling cascade to promote cell dedifferentiation might be conserved in at least several species of Brassicaceae and Solanaceae. PMID: 24389814
  2. The AP2/ERF transcription factor WIND1 controls cell dedifferentiation in Arabidopsis. [WIND1] [WOUND INDUCED DEDIFFERENTIATION 1] PMID: 21396822
  3. WIND1 is induced by wounding and promotes cell dedifferentiation. WIND proteins are required to activate the local cytokinin response at the wound site. PMID: 22112447
  4. Rap2.4f is a positive regulator of senescence, promoting both developmental and dark-induced leaf senescence. PMID: 20953945
Subcellular Location
Nucleus.
Protein Families
AP2/ERF transcription factor family, ERF subfamily
Tissue Specificity
Mostly expressed in roots and leaves. Also detected in flowers and stems.
Database Links

KEGG: ath:AT1G78080

STRING: 3702.AT1G78080.1

UniGene: At.25524

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