Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor WIN1(WIN1)

Code CSB-YP893141DOA
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Source Yeast
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Code CSB-EP893141DOA
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Source E.coli
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Code CSB-EP893141DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP893141DOA
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Source Baculovirus
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Code CSB-MP893141DOA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names WIN1
Uniprot No. Q9XI33
Alternative Names WIN1; ERF001; SHN1; At1g15360; F9L1.31Ethylene-responsive transcription factor WIN1; Protein SHINE 1; Protein WAX INDUCER 1
Species Arabidopsis thaliana (Mouse-ear cress)
Expression Region 1-199
Target Protein Sequence MVQTKKFRGV RQRHWGSWVA EIRHPLLKRR IWLGTFETAE EAARAYDEAA VLMSGRNAKT NFPLNNNNTG ETSEGKTDIS ASSTMSSSTS SSSLSSILSA KLRKCCKSPS PSLTCLRLDT ASSHIGVWQK RAGSKSDSSW VMTVELGPAS SSQETTSKAS QDAILAPTTE VEIGGSREEV LDEEEKVALQ MIEELLNTN
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Promotes cuticle formation by inducing the expression of enzymes involved in wax biosynthesis. Confers drought resistance. Acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways.
Gene References into Functions
  1. Overexpression of AtSHN1/WIN1 provokes unique defense responses. PMID: 23922943
  2. when expression of the WIN1/SHN1 gene was induced there was increased expression of genes involved in wax development... PMID: 22078383
  3. At least one of the cutin pathway genes, which encodes long-chain acyl-CoA synthetase LACS2, is likely to be directly targeted by WIN1. PMID: 17449808
  4. Work identified WIN1, a putative acetylornithine transaminase, as Pseudomonas syringae HopW1-1-interacting plant proteins. PMID: 18266921
Subcellular Location Nucleus.
Protein Families AP2/ERF transcription factor family, ERF subfamily
Tissue Specificity Expressed in aerial organs, mostly in flowers, and in roots. Also observed at the branch points of pedicels of most young flowers, and in a patchy pattern in roots of mature plants and very young leaves in the rosette, including support cells of their tri
Database Links

KEGG: ath:AT1G15360

STRING: 3702.AT1G15360.1

UniGene: At.11422

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