Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor WRI1(WRI1)

Code CSB-YP740965DOA
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Source Yeast
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Code CSB-EP740965DOA
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Source E.coli
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Code CSB-EP740965DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP740965DOA
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Source Baculovirus
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Code CSB-MP740965DOA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names WRI1
Uniprot No. Q6X5Y6
Alternative Names
WRI1; ASML1; At3g54320; T12E18.10; T12E18.20; Ethylene-responsive transcription factor WRI1; Protein ACTIVATOR OF SPORAMIN::LUC 1; Protein WRINKLED 1
Species Arabidopsis thaliana (Mouse-ear cress)
Expression Region 1-430
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways. Transcriptional activator involved in the activation of a subset of sugar-responsive genes and the control of carbon flow from sucrose import to oil accumulation in developing seeds. Binds to the GCC-box pathogenesis-related promoter element. Promotes sugar uptake and seed oil accumulation by glycolysis. Required for embryo development, seed germination and, indirectly, for seedling establishment. Negative regulator of the ABA-mediated germination inhibition.
Gene References into Functions
  1. MED15 is a target of WRI1. PMID: 27246098
  2. WRINKLED1 transcription factor role in the auxin homeostasis in roots PMID: 28981783
  3. WRI1 and WRI1-regulated genes involved in fatty acid synthesis were up-regulated, providing for a corresponding increase in the rate of fatty acid synthesis. PMID: 27208047
  4. The present study describes a novel metabolic engineering ploy involving the constitutive down-regulation of endogenous ADP-glucose pyrophosphorylase (BjAGPase) enzyme and the seed-specific expression of WRINKLED1 transcription factor (AtWRI1) from Arabidopsis thaliana in Indian mustard. PMID: 27314514
  5. A C-terminal intrinsically disordered region of WRINKLED1 is important for protein stability.WRINKLED1 plays role in oil accumulation. PMID: 26305482
  6. the structure and function of the WRI1 plant transcription factor, was investigated. PMID: 23922666
  7. Data show that CUL3 and BPM proteins assemble in planta with WRI1. PMID: 23792371
  8. WRI1 activates fatty acid biosynthesis in seeds for triacylglycerol production. WRI1, WRI3 and WRI4 are required in floral tissues to provide acyl chains for cutin synthesis and prevent adherence of these developing organs and subsequent semisterility. PMID: 23243127
  9. directly controls AtGLB1 expression PMID: 21070409
  10. WRI1 is able to regulate in planta the activity of the BCCP2 and PKp-beta1 promoters to enhance the transcription level of glycolytic and fatty acid biosynthetic genes. PMID: 19719479
  11. WRI1 promotes the flow of carbon to oil during seed maturation by directly activating genes involved in fatty acid synthesis and controlling genes for assembly and storage of triacylglycerol. PMID: 19594710
  12. The putative AP2/EREBP transcription factor WRINKLED1 (WRI1) is involved in the regulation of seed storage metabolism in Arabidopsis. [WRI1] PMID: 15500472
  13. ASML1/WRI1 is a transcriptional activator involved in the activation of a subset of sugar-responsive genes and the control of carbon flow from sucrose import to oil accumulation in developing seeds [ASML1][WRI1] [WRINKLED1] PMID: 15753106
  14. WRI1 is a target of LEAFY COTYLEDON2 and is necessary for the regulatory action on fatty acid metabolism. PMID: 17419836

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Subcellular Location Nucleus.
Protein Families AP2/ERF transcription factor family, AP2 subfamily
Tissue Specificity Mostly expressed in siliques, especially in seeds. Also detected in roots and flowers, and, to a lower extent, in leaves stems and seedlings.
Database Links

KEGG: ath:AT3G54320

STRING: 3702.AT3G54320.1

UniGene: At.35116

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