Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) WRI1 Polyclonal antibody

Uniprot No.

Q6X5Y6

Target Names

WRI1

Alternative Names

WRI1 antibody; ASML1 antibody; At3g54320 antibody; T12E18.10 antibody; T12E18.20 antibody; Ethylene-responsive transcription factor WRI1 antibody; Protein ACTIVATOR OF SPORAMIN::LUC 1 antibody; Protein WRINKLED 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana WRI1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways. Transcriptional activator involved in the activation of a subset of sugar-responsive genes and the control of carbon flow from sucrose import to oil accumulation in developing seeds. Binds to the GCC-box pathogenesis-related promoter element. Promotes sugar uptake and seed oil accumulation by glycolysis. Required for embryo development, seed germination and, indirectly, for seedling establishment. Negative regulator of the ABA-mediated germination inhibition.

Gene References into Functions

MED15 is a target of WRI1. PMID: 27246098
WRINKLED1 transcription factor role in the auxin homeostasis in roots PMID: 28981783
WRI1 and WRI1-regulated genes involved in fatty acid synthesis were up-regulated, providing for a corresponding increase in the rate of fatty acid synthesis. PMID: 27208047
The present study describes a novel metabolic engineering ploy involving the constitutive down-regulation of endogenous ADP-glucose pyrophosphorylase (BjAGPase) enzyme and the seed-specific expression of WRINKLED1 transcription factor (AtWRI1) from Arabidopsis thaliana in Indian mustard. PMID: 27314514
A C-terminal intrinsically disordered region of WRINKLED1 is important for protein stability.WRINKLED1 plays role in oil accumulation. PMID: 26305482
the structure and function of the WRI1 plant transcription factor, was investigated. PMID: 23922666
Data show that CUL3 and BPM proteins assemble in planta with WRI1. PMID: 23792371
WRI1 activates fatty acid biosynthesis in seeds for triacylglycerol production. WRI1, WRI3 and WRI4 are required in floral tissues to provide acyl chains for cutin synthesis and prevent adherence of these developing organs and subsequent semisterility. PMID: 23243127
directly controls AtGLB1 expression PMID: 21070409
WRI1 is able to regulate in planta the activity of the BCCP2 and PKp-beta1 promoters to enhance the transcription level of glycolytic and fatty acid biosynthetic genes. PMID: 19719479
WRI1 promotes the flow of carbon to oil during seed maturation by directly activating genes involved in fatty acid synthesis and controlling genes for assembly and storage of triacylglycerol. PMID: 19594710
The putative AP2/EREBP transcription factor WRINKLED1 (WRI1) is involved in the regulation of seed storage metabolism in Arabidopsis. [WRI1] PMID: 15500472
ASML1/WRI1 is a transcriptional activator involved in the activation of a subset of sugar-responsive genes and the control of carbon flow from sucrose import to oil accumulation in developing seeds [ASML1][WRI1] [WRINKLED1] PMID: 15753106
WRI1 is a target of LEAFY COTYLEDON2 and is necessary for the regulatory action on fatty acid metabolism. PMID: 17419836

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Subcellular Location

Nucleus.

Protein Families

AP2/ERF transcription factor family, AP2 subfamily

Tissue Specificity

Mostly expressed in siliques, especially in seeds. Also detected in roots and flowers, and, to a lower extent, in leaves stems and seedlings.

Database Links

KEGG: ath:AT3G54320

STRING: 3702.AT3G54320.1

UniGene: At.35116

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WRI1 Antibody

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