Recombinant Arabidopsis thaliana Eukaryotic initiation factor 4A-1 (TIF4A-1)

Code CSB-YP334836DOA
MSDS
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Source Yeast
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Code CSB-EP334836DOA
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Source E.coli
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Code CSB-EP334836DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP334836DOA
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Source Baculovirus
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Code CSB-MP334836DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TIF4A-1
Uniprot No.
Alternative Names
EIF4A1; RH4; TIF4A-1; At3g13920; MDC16.4Eukaryotic initiation factor 4A-1; eIF-4A-1; EC 3.6.4.13; ATP-dependent RNA helicase eIF4A-1; DEAD-box ATP-dependent RNA helicase 4; AtRH04
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-412
Target Protein Sequence
MAGSAPEGTQ FDARQFDQKL NEVLEGQDEF FTSYDDVHES FDAMGLQENL LRGIYAYGFE KPSAIQQRGI VPFCKGLDVI QQAQSGTGKT ATFCSGVLQQ LDFSLIQCQA LVLAPTRELA QQIEKVMRAL GDYLGVKVHA CVGGTSVRED QRILQAGVHV VVGTPGRVFD MLKRQSLRAD NIKMFVLDEA DEMLSRGFKD QIYDIFQLLP PKIQVGVFSA TMPPEALEIT RKFMSKPVRI LVKRDELTLE GIKQFYVNVE KEEWKLETLC DLYETLAITQ SVIFVNTRRK VDWLTDKMRS RDHTVSATHG DMDQNTRDII MREFRSGSSR VLITTDLLAR GIDVQQVSLV INFDLPTQPE NYLHRIGRSG RFGRKGVAIN FVTRDDERML FDIQKFYNVV VEELPSNVAD LL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Gene References into Functions
  1. Cyclin-dependent kinase A (CDKA) phosphorylates eukaryotic initiation factor 4A (eIF4A)eIF4A1 and eIF4A2 on a conserved threonine residue (threonine-164) within the RNA-binding motif. PMID: 27388680
  2. EIF4A function is essential for plant growth and development in Arabidopsis. PMID: 26493293
Subcellular Location
Cytoplasm.
Protein Families
DEAD box helicase family, eIF4A subfamily
Tissue Specificity
Highly expressed in the whole plant.
Database Links

KEGG: ath:AT3G13920

UniGene: At.23558

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