Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

EXPA1

Uniprot No.

Q9C554

Research Area

Others

Alternative Names

(AtEXPA1)(Alpha-expansin-1)(At-EXP1)(AtEx1)(Ath-ExpAlpha-1.2)

Species

Arabidopsis thaliana (Mouse-ear cress)

Source

E.coli

Expression Region

18-250aa

Target Protein Sequence

HVNGYAGGGWVNAHATFYGGGDASGTMGGACGYGNLYSQGYGTNTAALSTALFNNGLSCGACFEIRCQNDGKWCLPGSIVVTATNFCPPNNALPNNAGGWCNPPQQHFDLSQPVFQRIAQYRAGIVPVAYRRVPCVRRGGIRFTINGHSYFNLVLITNVGGAGDVHSAMVKGSRTGWQAMSRNWGQNWQSNSYLNGQSLSFKVTTSDGQTIVSNNVANAGWSFGQTFTGAQLR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

32.2 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Recombinant Arabidopsis thaliana (Mouse-ear cress) EXPA1 protein expression is initiated by transfecting e.coli cells with a DNA expression vector containing the gene encoding the protein of interest (18-250aa). The subsequent culturing of the cells induces the expression of the desired protein. The protein is equipped with a N-terminal 10xHis tag and C-terminal Myc tag. The resulting recombinant Arabidopsis thaliana (Mouse-ear cress) EXPA1 protein is collected and purified from the cell lysate through affinity purification, demonstrating a purity greater than 90%, as indicated by SDS-PAGE.

Target Background

Function

Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.

Gene References into Functions

AtEXPA1, regulates stomatal opening by altering the structure of the guard cell wall. PMID: 21593593
Data suggests a putative role for a guard cell expansin in the regulation of stomatal movement. [AtEXPA1] PMID: 20976459

Subcellular Location

Secreted, cell wall. Membrane; Peripheral membrane protein.

Protein Families

Expansin family, Expansin A subfamily

Tissue Specificity

Expressed in stomatal guard cells and very young vascular bundles throughout the plant.

Database Links

KEGG: ath:AT1G69530

STRING: 3702.AT1G69530.3

UniGene: At.23180

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Code	CSB-EP874889DOA
Abbreviation	Recombinant Mouse-ear cress EXPA1 protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Arabidopsis thaliana Expansin-A1 (EXPA1)

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