Recombinant Arabidopsis thaliana Extensin-1 (EXT1)

Code CSB-YP653631DOA
MSDS
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Source Yeast
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Code CSB-EP653631DOA
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Source E.coli
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Code CSB-EP653631DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP653631DOA
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Source Baculovirus
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Code CSB-MP653631DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
EXT1
Uniprot No.
Alternative Names
EXT1; EXT1/4; EXT4; At1g76930; F22K20.3Extensin-1; AtExt1; AtExt4; Extensin-1/4
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
20-373
Target Protein Sequence
N YFYSSPPPPV KHYSPPPVYK SPPPPVKHYS PPPVYKSPPP PVKHYSPPPV YKSPPPPVKY YSPPPVYKSP PPPVYKSPPP PVKHYSPPPV YKSPPPPVKH YSPPPVYKSP PPPVKHYSPP PVYKSPPPPV KHYSPPPVYK SPPPPVKYYS PPPVYKSPPP PVKHYSPPPV YKSPPPPVKY YSPPPVYKSP PPPVKHYSPP PVYKSPPPPV KYYSPPPVYK SPPPPVHYSP PPVVYHSPPP PVHYSPPPVV YHSPPPPVHY SPPPVVYHSP PPPVHYSPPP VVYHSPPPPV HYSPPPVVYH SPPPPKKHYE YKSPPPPVHY SPPTVYHSPP PPVHHYSPPH QPYLYKSPPP PHY
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Structural component which strengthens the primary cell wall.
Gene References into Functions
  1. Histochemical analysis of transgenic tobacco plants harboring the promoter of the Arabidopsis extensin gene atExt1 fused to the beta-glucuronidase gene coding sequence demonstrated expression of the transgene in stem tissues. PMID: 22869539
Subcellular Location
Secreted, primary cell wall.
Protein Families
Extensin family
Tissue Specificity
Predominantly expressed in the roots. Not detected in the leaves, nor in flowers or flower buds. Wounding reverses this pattern, turning on the gene in the leaves and repressing it in the roots.
Database Links

KEGG: ath:AT1G76930

STRING: 3702.AT1G76930.1

UniGene: At.20244

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