Recombinant Arabidopsis thaliana F-box protein GID2 (GID2)

Code CSB-YP890438DOA
MSDS
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Source Yeast
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Code CSB-EP890438DOA
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Source E.coli
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Code CSB-EP890438DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP890438DOA
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Source Baculovirus
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Code CSB-MP890438DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GID2
Uniprot No.
Alternative Names
GID2; SLY1; At4g24210; T22A6.40F-box protein GID2; Protein SLEEPY 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-151
Target Protein Sequence
MKRSTTDSDL AGDAHNETNK KMKSTEEEEI GFSNLDENLV YEVLKHVDAK TLAMSSCVSK IWHKTAQDER LWELICTRHW TNIGCGQNQL RSVVLALGGF RRLHSLYLWP LSKPNPRARF GKDELKLTLS LLSIRYYEKM SFTKRPLPES K
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Essential component of the SCF-type E3 ligase complex, SCF(GID2), a complex that positively regulates the gibberellin signaling pathway. Upon gibberellin treatment, the SCF(GID2) complex mediates the ubiquitination and subsequent degradation of DELLA proteins (GAI, RGA and RGL2), some repressors of the gibberellin pathway, leading to activate the pathway.
Gene References into Functions
  1. results indicate that, in Arabidopsis, AtSIZ1 positively controls GA signalling through SLY1 sumoylation. PMID: 26008766
  2. SNE function partly overlaps with SLY1 function in gibberellin signaling. PMID: 21163960
  3. SLY1 interacts directly with RGA and GA INSENSITIVE (GAI, a closely related DELLA protein) via their C-terminal GRAS domain PMID: 15155881
  4. SLY1 (the wild-type form) and sly1gar2-1 both confer substrate specificity on this complex via specific binding to the DELLA proteins PMID: 15161962
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in all tissues tested, including rosette leaves, green siliques, flowers, stems, cauline leaves and seedlings.
Database Links

KEGG: ath:AT4G24210

STRING: 3702.AT4G24210.1

UniGene: At.43332

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