Recombinant Arabidopsis thaliana F-box/LRR-repeat protein 17 (FBL17), partial

Code CSB-YP819826DOA
MSDS
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Source Yeast
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Code CSB-EP819826DOA
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Source E.coli
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Code CSB-EP819826DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP819826DOA
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Source Baculovirus
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Code CSB-MP819826DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FBL17
Uniprot No.
Alternative Names
FBL17; At3g54650; T5N23.10F-box/LRR-repeat protein 17; F-box-like protein 17
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Essential protein for male fertility. Component of the SCF(ASK-cullin-F-box) E3 ubiquitin ligase complex SCF(FBL17), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Enables the switch in cell cycle control leading to male germ cell lineage formation from microspores after meiosis. Targets CDKA-1 inhibitors the degradation specifically in male germ cells (e.g. KRP6 and KRP7) and thus enables CDKA-1 activation and germ cell S-phase progression. Promotes twin sperm cell production and double fertilization.
Gene References into Functions
  1. F-box protein FBL17 acts as a master cell cycle regulator during the diploid sporophyte phase of the plant. PMID: 25944099
  2. FBL17 is involved in cell cycle regulation during male gametogenesis. Loss-of-function mutants fail to undergo pollen mitosis II. PMID: 19277118
Subcellular Location
Nucleus. Note=Confined to the male germ cell nucleus of early to mid bicellular pollen.
Tissue Specificity
Expressed in developing pollen.
Database Links

KEGG: ath:AT3G54650

STRING: 3702.AT3G54650.1

UniGene: At.1208

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