Recombinant Arabidopsis thaliana FHA domain-containing protein DDL (DDL)

Code CSB-YP823826DOA
MSDS
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Source Yeast
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Code CSB-EP823826DOA
MSDS
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Source E.coli
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Code CSB-EP823826DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP823826DOA
MSDS
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Source Baculovirus
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Code CSB-MP823826DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DDL
Uniprot No.
Alternative Names
DDL; At3g20550; K10D20.9; FHA domain-containing protein DDL; Protein DAWDLE
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-314
Target Protein Sequence
MAPSSRSPSP RTKRLRRARG EKEIGRSRER EDDGREREKR NSRERDRDIG RDRDRERKGE GERDREVGDK RRRSGREDTE KRRRTRTDDE RYSRGRHERS TSPSDRSHRS SRRSPERAIA SRHDEGSNAR GGSEEPNVEE DSVARMRAVE EALAAKKKEE PSFELSGKLA EETNRYRGIT LLFNEPPEAR KPSERWRLYV FKDGEPLNEP LCLHRQSCYL FGRERRIADI PTDHPSCSKQ HAVIQYREME KEKPDGMMGK QVKPYIMDLG STNKTYINES PIEPQRYYEL FEKDTIKFGN SSREYVLLHE NSAE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the microRNA (miRNA) and short interfering RNA (siRNA) biogenesis. May facilitate DCL1 to access or recognize primary miRNAs. Binds RNA non-specifically.
Gene References into Functions
  1. identified DDL as a physiological target of protein PARylation in plant immunity, which is uncoupled from its function in plant development PMID: 27797852
  2. the recognition of the target residue by the canonical binding cleft of the DDL FHA domain as the key molecular event to instate FHA domain-mediated protein-protein interaction in plant miRNA processing PMID: 23313986
  3. DDL is expressed in the root and shoot meristems and the reduced size of the root apical meristem in ddl plants suggests a role early in organ development. PMID: 16679419
  4. propose that DDL participates in miRNA biogenesis by facilitating DCL1 to access or recognize pri-miRNAs PMID: 18632581
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in roots, lateral roots, vascular strands of roots and leaves, vegetative meristems, pollen and developing seeds.
Database Links

KEGG: ath:AT3G20550

STRING: 3702.AT3G20550.1

UniGene: At.27430

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