Recombinant Arabidopsis thaliana Ferredoxin--NADP reductase, leaf isozyme 1, chloroplastic (LFNR1)

Code CSB-YP887793DOA
MSDS
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Source Yeast
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Code CSB-EP887793DOA
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Source E.coli
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Code CSB-EP887793DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887793DOA
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Source Baculovirus
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Code CSB-MP887793DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LFNR1
Uniprot No.
Alternative Names
LFNR1; PETH1; At5g66190; K2A18.27Ferredoxin--NADP reductase; leaf isozyme 1; chloroplastic; EC 1.18.1.2; Leaf FNR 1; AtLFNR1; FNR-1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
50-360
Target Protein Sequence
V TTDTTEAPPV KVVKESKKQE EGIVVNKFKP KNPYTGRCLL NTKITGDDAP GETWHIVFTT EGEVPYREGQ SIGVIPEGID KNGKPHKLRL YSIASSAIGD FGDSKTVSLC VKRLVYTNDG GEIVKGVCSN FLCDLKPGDE AKITGPVGKE MLMPKDPNAT IIMLGTGTGI APFRSFLWKM FFEEHEDYKF NGLAWLFLGV PTSSSLLYKE EFEKMKEKNP DNFRLDFAVS REQTNEKGEK MYIQTRMAEY AEELWELLKK DNTFVYMCGL KGMEKGIDDI MVSLAAKDGI DWLEYKKQLK RSEQWNVEVY
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power.
Gene References into Functions
  1. fnr1 knock-outs, the NADP(H) and glutathione pools are more oxidized relative to the wild type, and the photoprotective effect is absent despite perception of oxidative stress at the level of gene transcription. PMID: 27634426
  2. critical player in nitrogen-regulated flowering time PMID: 27325772
  3. The dynamic, multilayer regulation of AtFNR exemplifies the complex regulatory network systems controlling chloroplast proteins by a range of posttranslational modifications. PMID: 25301888
  4. The depletion of FNR cause the downregulation of photosynthetic machinery and the induction of photoprotective mechanisms. PMID: 22300243
  5. FNR1 mutants showed an enhanced rate of cyclic electron transfer under normal growth conditions. PMID: 19054362

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Subcellular Location
Plastid, chloroplast stroma. Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Stromal side.
Protein Families
Ferredoxin--NADP reductase type 1 family
Tissue Specificity
Expressed in shoots. Restricted to green tissues, being more abundant in siliques.
Database Links

KEGG: ath:AT5G66190

STRING: 3702.AT5G66190.1

UniGene: At.47570

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