Recombinant Arabidopsis thaliana Ferritin-1, chloroplastic (FER1)

Code CSB-YP658578DOA
MSDS
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Source Yeast
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Code CSB-EP658578DOA
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Source E.coli
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Code CSB-EP658578DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP658578DOA
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Source Baculovirus
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Code CSB-MP658578DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FER1
Uniprot No.
Alternative Names
FER1; At5g01600; F7A7.120Ferritin-1; chloroplastic; AtFer1; EC 1.16.3.1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
49-255
Target Protein Sequence
AA TVDTNNMPMT GVVFQPFEEV KKADLAIPIT SHASLARQRF ADASEAVINE QINVEYNVSY VYHSMYAYFD RDNVAMKGLA KFFKESSEEE RGHAEKFMEY QNQRGGRVKL HPIVSPISEF EHAEKGDALY AMELALSLEK LTNEKLLNVH KVASENNDPQ LADFVESEFL GEQIEAIKKI SDYITQLRMI GKGHGVWHFD QMLLN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.
Gene References into Functions
  1. phosphate starvation response of AtFer1 is not linked to the iron status of plants and is specifically initiated by phosphate deficiency PMID: 23788639
  2. Data show that a leaf glutathione concentration threshold between 10 and 50 nmol GSHg(-1) FW is required for full induction of AtFer1 gene expression in response to iron. PMID: 22449975
  3. new regulatory pathway involved in plant response to oxidative stress was revealed using the iron-induced Arabidopsis ferritin AtFER1 as a model PMID: 21946558
  4. Ferritin accumulation during infection of Arabidopsis by E. chrysanthemi is a basal defense mechanism which is mainly activated by bacterial siderophores. PMID: 15998312
Subcellular Location
Plastid, chloroplast.
Protein Families
Ferritin family
Database Links

KEGG: ath:AT5G01600

STRING: 3702.AT5G01600.1

UniGene: At.23533

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