Recombinant Arabidopsis thaliana Flavin-containing monooxygenase YUCCA2 (YUC2)

Code CSB-YP882979DOA
MSDS
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Source Yeast
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Code CSB-EP882979DOA
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Source E.coli
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Code CSB-EP882979DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP882979DOA
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Source Baculovirus
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Code CSB-MP882979DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
YUC2
Uniprot No.
Alternative Names
YUC2; YUCCA2; At4g13260; F17N18.150; Indole-3-pyruvate monooxygenase YUCCA2; EC 1.14.13.168; Flavin-containing monooxygenase YUCCA2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-415
Target Protein Sequence
MEFVTETLGK RIHDPYVEET RCLMIPGPII VGSGPSGLAT AACLKSRDIP SLILERSTCI ASLWQHKTYD RLRLHLPKDF CELPLMPFPS SYPTYPTKQQ FVQYLESYAE HFDLKPVFNQ TVEEAKFDRR CGLWRVRTTG GKKDETMEYV SRWLVVATGE NAEEVMPEID GIPDFGGPIL HTSSYKSGEI FSEKKILVVG CGNSGMEVCL DLCNFNALPS LVVRDSVHVL PQEMLGISTF GISTSLLKWF PVHVVDRFLL RMSRLVLGDT DRLGLVRPKL GPLERKIKCG KTPVLDVGTL AKIRSGHIKV YPELKRVMHY SAEFVDGRVD NFDAIILATG YKSNVPMWLK GVNMFSEKDG FPHKPFPNGW KGESGLYAVG FTKLGLLGAA IDAKKIAEDI EVQRHFLPLA RPQHC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in auxin biosynthesis. Converts the indole-3-pyruvic acid (IPA) produced by the TAA family to indole-3-acetic acid (IAA). Unable to use tryptamine (TAM) as substrate. Required for the formation of floral organs and vascular tissues. Belongs to the set of redundant YUCCA genes probably responsible for auxin biosynthesis in shoots.
Gene References into Functions
  1. In BnaA.TTG2.a.1-overexpressing Arabidopsis under salt stress, the endogenous indole-3-acetic acid content was reduced, and expression of two auxin biosynthesis genes, TRYPTOPHAN BIOSYNTHESIS 5 (TRP5) and YUCCA2 (YUC2), was downregulated. PMID: 26071533
  2. Expressions of YUCs in the leaf respond to the adaxial-abaxial juxtaposition, and that the activities of auxin mediate leaf margin development, which subsequently promotes blade outgrowth. PMID: 22003085
  3. SPL regulates auxin homeostasis by repressing the transcription of YUC2 and YUC6 and participates in lateral organ morphogenesis. PMID: 18557819
Protein Families
FMO family
Tissue Specificity
Expressed in anthers and in the apical gynoecium.
Database Links

KEGG: ath:AT4G13260

STRING: 3702.AT4G13260.1

UniGene: At.33397

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