Recombinant Arabidopsis thaliana Flavin-containing monooxygenase YUCCA4 (YUC4)

Code CSB-YP878484DOA
MSDS
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Source Yeast
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Code CSB-EP878484DOA
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Source E.coli
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Code CSB-EP878484DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP878484DOA
MSDS
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Source Baculovirus
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Code CSB-MP878484DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
YUC4
Uniprot No.
Alternative Names
YUC4; THREAD; YUCCA4; At5g11320; F2I11_210; Probable indole-3-pyruvate monooxygenase YUCCA4; EC 1.14.13.168; Flavin-containing monooxygenase YUCCA4
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-411
Target Protein Sequence
MGTCRESEPT QIFVPGPIIV GAGPSGLAVA ACLSNRGVPS VILERTDCLA SLWQKRTYDR LKLHLPKHFC ELPLMPFPKN FPKYPSKQLF ISYVESYAAR FNIKPVFNQT VEKAEFDDAS GLWNVKTQDG VYTSTWLVVA TGENAEPVFP NIPGLKKFTG PVVHTSAYKS GSAFANRKVL VVGCGNSGME VSLDLCRYNA LPHMVVRNSV HVLPRDFFGL STFGIAMTLL KWFPLKLVDK FLLLLANSTL GNTDLLGLRR PKTGPIELKN VTGKTPVLDV GAISLIRSGQ IKVTQAVKEI TRNGAKFLNG KEIEFDSIIL ATGYKSNVPD WLKENSFFTK EGMPKTPFPN GWKGEKGLYT VGFTRRGLSG TAYDAVKIAE DITDQWMKFN GPLSCRNICS SHIIHLHFNK S
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in auxin biosynthesis. Both isoforms are catalitically active. Involved during embryogenesis and seedling development. Required for the formation of floral organs and vascular tissues. Belongs to the set of redundant YUCCA genes probably responsible for auxin biosynthesis in shoots.
Gene References into Functions
  1. Data show the differential localization of YUCCA4 (At5g11320) alternative splice variants to the endoplasmic reticulum and the cytosol, which depends on tissue-specific splicing. PMID: 22233288
Subcellular Location
[Isoform 1]: Cytoplasm.; [Isoform 2]: Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side.
Protein Families
FMO family
Tissue Specificity
Expressed in leaves, stems, flowers, buds and siliques. Detected in the apical gynoecium and in the developing ovules.
Database Links

KEGG: ath:AT5G11320

STRING: 3702.AT5G11320.1

UniGene: At.32275

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