Recombinant Arabidopsis thaliana Flavin-containing monooxygenase YUCCA6 (YUC6)

Code CSB-YP845073DOA
MSDS
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Source Yeast
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Code CSB-EP845073DOA
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Source E.coli
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Code CSB-EP845073DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP845073DOA
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Source Baculovirus
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Code CSB-MP845073DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
YUC6
Uniprot No.
Alternative Names
YUC6; HYT1; YUCCA6; At5g25620; T14C9.160; Indole-3-pyruvate monooxygenase YUCCA6; EC 1.14.13.168; Flavin-containing monooxygenase YUCCA6; Protein HYPERTALL1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-417
Target Protein Sequence
MDFCWKREME GKLAHDHRGM TSPRRICVVT GPVIVGAGPS GLATAACLKE RGITSVLLER SNCIASLWQL KTYDRLHLHL PKQFCELPII PFPGDFPTYP TKQQFIEYLE DYARRFDIKP EFNQTVESAA FDENLGMWRV TSVGEEGTTE YVCRWLVAAT GENAEPVVPR FEGMDKFAAA GVVKHTCHYK TGGDFAGKRV LVVGCGNSGM EVCLDLCNFG AQPSLVVRDA VHVLPREMLG TSTFGLSMFL LKWLPIRLVD RFLLVVSRFI LGDTTLLGLN RPRLGPLELK NISGKTPVLD VGTLAKIKTG DIKVCSGIRR LKRHEVEFDN GKTERFDAII LATGYKSNVP SWLKENKMFS KKDGFPIQEF PEGWRGECGL YAVGFTKRGI SGASMDAKRI AEDIHKCWKQ DEQVKKI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in auxin biosynthesis via the indole-3-pyruvic acid (IPA) pathway. Also able to convert in vitro phenyl pyruvate (PPA) to phenyl acetic acid (PAA). Required for the formation of floral organs and vascular tissues. Belongs to the set of redundant YUCCA genes probably responsible for auxin biosynthesis in shoots.
Gene References into Functions
  1. Arabidopsis plants overexpressing YUC6 display enhanced IAA-related phenotypes and exhibit improved drought stress tolerance, low rate of water loss and controlled ROS accumulation under drought and oxidative stresses. PMID: 26314500
  2. Transgenic poplar expressing Arabidopsis YUCCA6 exhibits auxin-overproduction phenotypes and increased tolerance to abiotic stress. PMID: 25980973
  3. YUCCA6 enhances drought tolerance through regulated reactive oxygen species homeostasis PMID: 23603963
  4. a functional YUCCA pathway of auxin biosynthesis in potato PMID: 22986790
  5. YUC6 uses NADPH and oxygen to convert indole-3-pyruvic acid to indole-3-acetic acid. PMID: 23188833
  6. Arabidopsis plants over-expressing YUCCA6 displayed dramatic longevity. In addition, plants over-expressing YUCCA6 exhibited classical, delayed dark-induced and hormone-induced senescence in leaves. PMID: 21511905
  7. results identify YUCCA6 as a functional member of the YUCCA family with unique roles in growth and development PMID: 17885085

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Subcellular Location
Cytoplasm.
Protein Families
FMO family
Tissue Specificity
Highly expressed in roots but modestly expressed in the cauline leaves and flowers. Expressed in anthers.
Database Links

KEGG: ath:AT5G25620

STRING: 3702.AT5G25620.2

UniGene: At.28557

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