Recombinant Arabidopsis thaliana G-type lectin S-receptor-like serine/threonine-protein kinase SD1-29 (SD129), partial

Code CSB-YP527710DOA
MSDS
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Source Yeast
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Code CSB-EP527710DOA
MSDS
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Source E.coli
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Code CSB-EP527710DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP527710DOA
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Source Baculovirus
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Code CSB-MP527710DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SD129
Uniprot No.
Alternative Names
SD129; At1g61380; T1F9.13; G-type lectin S-receptor-like serine/threonine-protein kinase SD1-29; EC 2.7.11.1; S-domain-1; SD1) receptor kinase 29; SD1-29
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
S-domain receptor protein kinase involved in lipopolysaccharide (LPS) sensing. Specifically detects LPS of Pseudomonas and Xanthomonas species. LPS are major components of the outer membrane of Gram-negative bacteria and are important microbe-associated molecular patterns (MAMPs) that trigger biphasic production of reactive oxygen species (ROS) and immune responses in plants. Seems to be only partially associated with the second LPS-triggered ROS burst. Mediates defense signaling in response to the medium-chain 3-hydroxy fatty acid 3-OH-C10:0, a pathogen-associated molecular pattern (PAMP) which induces autophosphorylation at Tyr-600. Autophosphorylation at Tyr-600 is required for downstream phosphorylation of the receptor-like cytoplasmic kinase PBL34, PBL35 and PBL36, and activation of plant immunity.; (Microbial infection) Targeted by the bacterial type III effector protein tyrosine phosphatase HopAO1 from Pseudomonas syringae. HopAO1 dephosphorylates Tyr-600, which suppresses the immune response.
Gene References into Functions
  1. SD1-29 mediates lipopolysaccharide sensing in Arabidopsis thaliana. PMID: 25729922
Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family
Database Links

KEGG: ath:AT1G61380

STRING: 3702.AT1G61380.1

UniGene: At.36467

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