Recombinant Arabidopsis thaliana GLABRA2 expression modulator (GEM)

Code CSB-YP837307DOA
MSDS
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Source Yeast
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Code CSB-EP837307DOA
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Source E.coli
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Code CSB-EP837307DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP837307DOA
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Source Baculovirus
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Code CSB-MP837307DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GEM
Uniprot No.
Alternative Names
GEM; At2g22475; F14M13.2GLABRA2 expression modulator
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-299
Target Protein Sequence
MEPPKGDTVV KTEVPVKDPS LSVVDSKTKG VEDANTEIAL SDEVEIETKG SDSTPVKAPS RTSSGSKKSV HWSPELVSGS QEPDQKAASS SSAGSNPYIA RSPAETSDAS LKDTMETVKG VLGRWGKRVA EAAKKTESLA GNTWQHLRTA PSFADAAMGR IAQSTKVFAE GGYEKIFRQT FETDPEEQLL NSFACYLSTS AGPVMGVLYI SSAKLAYCSD NPLSYKNGDQ TEWSYYKVVI PLHQLKAVNP SASIVNPAEK YIQVISVDNH EFWFMGFLNY DGAVTSLQDS LQAGALRSV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the spatial control of cell division, patterning and differentiation of Arabidopsis root epidermal cells. Could be part of a complex that negatively modulates GLABRA2 and CAPRICE expression via the maintenance of a repressor histone H3 epigenetics status of the GL2 and CPC promoters.
Gene References into Functions
  1. These data suggest that GEM is an abscisic acid-responsive protein that may function downstream of ABI5 as part of the abscisic acid signaling pathway. PMID: 26939893
  2. GL2 directly regulates genes with various hierarchies in epidermal cell differentiation pathways. PMID: 22943441
  3. GEM participates in the maintenance of the repressor histone H3K9 methylation status of root patterning genes, providing a link between cell division, fate and differentiation during Arabidopsis root development [GEM] PMID: 17450124
Protein Families
GEM family
Tissue Specificity
Ubiquitous.
Database Links

KEGG: ath:AT2G22475

STRING: 3702.AT2G22475.1

UniGene: At.19761

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