Recombinant Arabidopsis thaliana Gibberellin 20 oxidase 3 (20ox3)

Code CSB-YP655791DOA
MSDS
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Source Yeast
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Code CSB-EP655791DOA
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Source E.coli
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Code CSB-EP655791DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP655791DOA
MSDS
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Source Baculovirus
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Code CSB-MP655791DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GA20OX3
Uniprot No.
Alternative Names
GA20OX3; 20ox3; YAP169; At5g07200; T28J14_140Gibberellin 20 oxidase 3; EC 1.14.11.-; GA 20-oxidase 3; Gibberellin C-20 oxidase 3
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-380
Target Protein Sequence
MATECIATVP QIFSENKTKE DSSIFDAKLL NQHSHHIPQQ FVWPDHEKPS TDVQPLQVPL IDLAGFLSGD SCLASEATRL VSKAATKHGF FLITNHGVDE SLLSRAYLHM DSFFKAPACE KQKAQRKWGE SSGYASSFVG RFSSKLPWKE TLSFKFSPEE KIHSQTVKDF VSKKMGDGYE DFGKVYQEYA EAMNTLSLKI MELLGMSLGV ERRYFKEFFE DSDSIFRLNY YPQCKQPELA LGTGPHCDPT SLTILHQDQV GGLQVFVDNK WQSIPPNPHA FVVNIGDTFM ALTNGRYKSC LHRAVVNSER ERKTFAFFLC PKGEKVVKPP EELVNGVKSG ERKYPDFTWS MFLEFTQKHY RADMNTLDEF SIWLKNRRSF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Key oxidase enzyme in the biosynthesis of gibberellin that catalyzes the conversion of GA12 and GA53 to GA9 and GA20 respectively, via a three-step oxidation at C-20 of the GA skeleton. GA53 is less effectively oxidized than GA12, and GA25 is also formed as a minor product.
Gene References into Functions
  1. TSN is a novel component of plant stress granules (SGs) that regulates growth under salt stress by modulating levels of GA20ox3 mRNA. PMID: 25205572
Protein Families
Iron/ascorbate-dependent oxidoreductase family, GA20OX subfamily
Tissue Specificity
Expressed at high level in developing siliques. Detected in seeds, roots, leaves and inflorescences. In seeds, specifically detected at the outer layer of the outer integument.
Database Links

KEGG: ath:AT5G07200

STRING: 3702.AT5G07200.1

UniGene: At.5050

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