Recombinant Arabidopsis thaliana Gibberellin receptor GID1B (GID1B)

Code CSB-YP881861DOA
MSDS
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Source Yeast
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Code CSB-EP881861DOA
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Source E.coli
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Code CSB-EP881861DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP881861DOA
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Source Baculovirus
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Code CSB-MP881861DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GID1B
Uniprot No.
Alternative Names
GID1B; CXE14; GID1L2; At3g63010; T20O10.110Gibberellin receptor GID1B; EC 3.-.-.-; AtCXE14; Carboxylesterase 14; GID1-like protein 2; Protein GA INSENSITIVE DWARF 1B; AtGID1B
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-358
Target Protein Sequence
MAGGNEVNLN ECKRIVPLNT WVLISNFKLA YKVLRRPDGS FNRDLAEFLD RKVPANSFPL DGVFSFDHVD STTNLLTRIY QPASLLHQTR HGTLELTKPL STTEIVPVLI FFHGGSFTHS SANSAIYDTF CRRLVTICGV VVVSVDYRRS PEHRYPCAYD DGWNALNWVK SRVWLQSGKD SNVYVYLAGD SSGGNIAHNV AVRATNEGVK VLGNILLHPM FGGQERTQSE KTLDGKYFVT IQDRDWYWRA YLPEGEDRDH PACNPFGPRG QSLKGVNFPK SLVVVAGLDL VQDWQLAYVD GLKKTGLEVN LLYLKQATIG FYFLPNNDHF HCLMEELNKF VHSIEDSQSK SSPVLLTP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Functions as soluble gibberellin (GA) receptor. GA is an essential hormone that regulates growth and development in plants. Binds with high affinity the biologically active gibberellin GA4, but has no affinity for the biologically inactive GAs. In response to GA, interacts with specific DELLA proteins, known as repressors of GA-induced growth, and targets them for degradation via proteasome. Seems to be required for GA signaling that controls root growth, seed germination and flower development. May function as a dominant GA receptor at low GA concentrations in germination. Partially redundant with GID1A and GID1C.
Gene References into Functions
  1. The stamens of the double knockout mutant atgid1a atgid1b were significantly shorter than those of the wild-type, and this leads to low fertility. PMID: 17521411
  2. GID1a, GID1b, GID1c are the predominant GA receptors in regulating GA growth response in Arabidopsis. GA binding to GID1a promotes its interaction with DELLA proteins. [GID1b] PMID: 17194763
  3. AtGID1a, AtGID1b and AtGID1c function as GA receptors in Arabidopsis.[AtGID1b] PMID: 16709201
Subcellular Location
Nucleus.
Protein Families
'GDXG' lipolytic enzyme family
Tissue Specificity
Widely expressed.
Database Links

KEGG: ath:AT3G63010

STRING: 3702.AT3G63010.1

UniGene: At.47986

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