Recombinant Arabidopsis thaliana Gibberellin-regulated protein 5 (GASA5)

Code CSB-YP767993DOA
MSDS
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Source Yeast
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Code CSB-EP767993DOA
MSDS
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Source E.coli
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Code CSB-EP767993DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP767993DOA
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Source Baculovirus
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Code CSB-MP767993DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GASA5
Uniprot No.
Alternative Names
GASA5; At3g02885; F13E7Gibberellin-regulated protein 5; GAST1 protein homolog 5
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
28-97
Target Protein Sequence
ARG GGKLKPQQCN SKCSFRCSAT SHKKPCMFFC LKCCKKCLCV PPGTFGNKQT CPCYNNWKTK EGRPKCP
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Gibberellin-regulated protein that acts as a negative regulator of gibberellin-induced flowering and stem growth. May inhibit flowering and inflorescence growth via a pathway involving GAI and by enhancing FLC expression and repressing FT and LFY. Acts as a negative regulator in thermotolerance by resogulating both salicylic acid (SA) signaling and heat shock-protein accumulation.
Gene References into Functions
  1. GASA5 suppresses gibberellin responses in Arabidopsis PMID: 24157610
  2. The Arabidopsis GASA5 gene acts as a negative regulator in thermotolerance by regulating both salicylic acid signaling and heat shock-protein accumulation. PMID: 21835493
  3. Results suggest that GASA5 is a negative regulator of GA-induced flowering and stem growth. PMID: 19190987
Subcellular Location
Secreted. Secreted, cell wall. Secreted, extracellular space, extracellular matrix.
Protein Families
GASA family
Tissue Specificity
Expressed in roots, root hairs, vasculature of cotyledons and hypocotyls, shoot apex, leaf veins, stems, flower receptacles, pollen, filaments, anthers and siliques.
Database Links

KEGG: ath:AT3G02885

STRING: 3702.AT3G02885.1

UniGene: At.5431

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