Recombinant Arabidopsis thaliana Glutamate--glyoxylate aminotransferase 1 (GGAT1)

Code CSB-YP888781DOA
MSDS
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Source Yeast
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Code CSB-EP888781DOA
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Source E.coli
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Code CSB-EP888781DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP888781DOA
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Source Baculovirus
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Code CSB-MP888781DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GGAT1
Uniprot No.
Alternative Names
GGAT1; AOAT1; GGT1; At1g23310; F26F24.16; F26F24_4Glutamate--glyoxylate aminotransferase 1; AtGGT2; EC 2.6.1.4; Alanine aminotransferase GGT1; EC 2.6.1.2; Alanine--glyoxylate aminotransferase GGT1; EC 2.6.1.44; Alanine-2-oxoglutarate aminotransferase 1; EC 2.6.1.-
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-481
Target Protein Sequence
MALKALDYDT LNENVKKCQY AVRGELYLRA SELQKEGKKI IFTNVGNPHA LGQKPLTFPR QVVALCQAPF LLDDPNVGML FPADAIARAK HYLSLTSGGL GAYSDSRGLP GVRKEVAEFI QRRDGYPSDP ELIFLTDGAS KGVMQILNCV IRGNGDGILV PVPQYPLYSA TISLLGGTLV PYYLDESENW GLDVANLRQS VAQARSQGIT VRAMVIINPG NPTGQCLSEA NIREILKFCY NEKLVLLGDE VYQQNIYQDE RPFISSKKVL MEMGSPFSKE VQLVSFHTVS KGYWGECGQR GGYFEMTNLP PRVVEEIYKV ASIALSPNVS AQIFMGLMVN PPKPGDISYD QFARESKGIL ESLRRRARLM TDGFNSCKNV VCNFTEGAMY SFPQIRLPTG ALQAAKQAGK VPDVFYCLKL LEATGISTVP GSGFGQKEGV FHLRTTILPA EDEMPEIMDS FKKFNDEFMT QYDNNFGYSK M
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the glutamate:glyoxylate (GGT or GGAT), alanine:glyoxylate (AGT), alanine:2-oxoglutarate (AKT) and glutamate:pyruvate (GPT) aminotransferase reactions in peroxisomes. Required for abscisic acid (ABA)- and stress-mediated responses in an H(2)O(2)-dependent manner. Functions as a photorespiratory aminotransferase that modulates amino acid content during photorespiration (GGAT activity); promotes serine, glycine and citrulline metabolism in response to light.
Gene References into Functions
  1. GGT1 protein plays a role in maintaining leaf carbon/nitrogen balance by reducing RuBisCO content and plant growth. PMID: 26216646
  2. These observations suggest that GGT1 is important in preventing oxidative stress by metabolizing extracellular glutathione disulfide, while GGT2 might be important in transporting glutathione into developing seeds. PMID: 17316175
  3. Mutations in GGT1 result in altered abscisic acid sensitivity. PMID: 17318317
Subcellular Location
Peroxisome.
Protein Families
Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily
Tissue Specificity
Mostly expressed in leaves, and, to a lower extent, in shoots, stems, flowers, seedlings and green siliques.
Database Links

KEGG: ath:AT1G23310

STRING: 3702.AT1G23310.1

UniGene: At.24749

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