Recombinant Arabidopsis thaliana Histone deacetylase 14 (HDA14)

Code CSB-YP856692DOA
MSDS
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Source Yeast
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Code CSB-EP856692DOA
MSDS
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Source E.coli
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Code CSB-EP856692DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP856692DOA
MSDS
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Source Baculovirus
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Code CSB-MP856692DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HDA14
Uniprot No.
Alternative Names
HDA14; At4g33470; F17M5.230; Histone deacetylase 14; EC 3.5.1.98
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-423
Target Protein Sequence
MSMALIVRPF FVPGSAGISG SRNICKKNQW RKYLLKPSGS SINCSFSTEK NPLLPSIQQL ADARLIYSVS AALGHNKESH PECSARVPAI VNALEMNELT PKFRGSQILE LANFKTATVE DIANVHDKAY VFGLEKAMDE ASDSGLIFIE GSGPTYATST TFQDSLIAAG AGMALVDSVI AASRNSVDPP IGFALIRPPG HHAVPKGPMG FCVFGNVAIA ARHAQRTHGL KRIFIIDFDV HHGNGTNDAF TEDPDIFFLS THQDGSYPGT GKISDIGKGK GEGTTLNLPL PGGSGDIAMR TVFEEIIVPC AQRFKPDIIL VSAGYDAHVL DPLANLQFTT ATYYSLAKDI KRLAKEVCGG RCVFFLEGGY NLESLSSSVA DSFRALLGED SLASEFDNPA YLYDEPMRKV RDAIQRAKSI HCL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulates lysine acetylation levels of plastid proteins related to photosynthesis. Involved in the regulation of the activation state of RuBisCO, which is controlled by lysine acetylation of RuBisCO activase under low-light conditions. Associates with alpha- and beta-tubulins and deacetylate alpha-tubulin. Does not seem to be required for the cellular patterning in the root epidermis. Involved in the regulation of melatonin biosynthesis by catalyzing the deacetylation of N-acetylserotonin to produce serotonin. N-acetylserotonin is methylated by acetylserotonin O-methyltransferase (ASMT) to produce melatonin (N-acetyl-5-methoxytryptamine) (Probable). Deacetylates melatonin to produce 5-methoxytryptamine. In vitro, deacetylates N-acetyltyramine and N-acetyltryptamine to produce tyramine and tryptamine, respectively.
Gene References into Functions
  1. HDA14, ELP3 and the PP2A A-subunits A1, A2 and A3 all reside in both the nucleus and cytosol of the cell. PMID: 22404109
Subcellular Location
Nucleus. Cytoplasm. Plastid, chloroplast stroma. Mitochondrion.
Protein Families
Histone deacetylase family
Tissue Specificity
Expressed in stems, leaves, flowers, siliques and mature seeds.
Database Links

KEGG: ath:AT4G33470

STRING: 3702.AT4G33470.1

UniGene: At.26427

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