Recombinant Arabidopsis thaliana Histone deacetylase HDT1 (HDT1)

Code CSB-YP872327DOA
MSDS
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Source Yeast
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Code CSB-EP872327DOA
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Source E.coli
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Code CSB-EP872327DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP872327DOA
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Source Baculovirus
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Code CSB-MP872327DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HDT1
Uniprot No.
Alternative Names
HDT1; HD2A; HDA3; At3g44750; T32N15.8; Histone deacetylase HDT1; HD-tuins protein 1; Histone deacetylase 2a
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-245
Target Protein Sequence
MEFWGIEVKS GKPVTVTPEE GILIHVSQAS LGECKNKKGE FVPLHVKVGN QNLVLGTLST ENIPQLFCDL VFDKEFELSH TWGKGSVYFV GYKTPNIEPQ GYSEEEEEEE EEVPAGNAAK AVAKPKAKPA EVKPAVDDEE DESDSDGMDE DDSDGEDSEE EEPTPKKPAS SKKRANETTP KAPVSAKKAK VAVTPQKTDE KKKGGKAANQ SPKSASQVSC GSCKKTFNSG NALESHNKAK HAAAK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Probably mediates the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Required for histone H3 'Lys-9' deacetylation. Involved in rRNA gene silencing in nucleolar dominance. Seems to be implicated in the regulation of genes involved in seeds development.
Gene References into Functions
  1. These results suggest that by repressing the expression of GA2ox2, HDT1/2 likely fine-tune gibberellin metabolism. PMID: 28855334
  2. HD2A and HD2C may have different and opposing functions in germination with the glucose/HD2A pathway acting to restrain germination and the HD2C pathway acting to enhance germination. PMID: 21739146
Subcellular Location
Nucleus, nucleolus.
Protein Families
Histone deacetylase HD2 family
Tissue Specificity
Expressed in leaves, roots, stems, young plantlets, flowers and siliques. Highest levels in ovules, embryos, shoot apical meristems and first leaves. Also expressed in somatic embryos.
Database Links

KEGG: ath:AT3G44750

STRING: 3702.AT3G44750.1

UniGene: At.20950

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