Recombinant Arabidopsis thaliana Histone-lysine N-methyltransferase SUVR4 (SUVR4)

Code CSB-YP823832DOA
MSDS
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Source Yeast
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Code CSB-EP823832DOA
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Source E.coli
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Code CSB-EP823832DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP823832DOA
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Source Baculovirus
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Code CSB-MP823832DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SUVR4
Uniprot No.
Alternative Names
SUVR4; SDG31; SET31; At3g04380; T27C4.2; Histone-lysine N-methyltransferase SUVR4; EC 2.1.1.-; Protein SET DOMAIN GROUP 31; Suppressor of variegation 3-9-related protein 4; Su(var)3-9-related protein 4
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-492
Target Protein Sequence
MISLSGLTSS VESDLDMQQA MLTNKDEKVL KALERTRQLD IPDEKTMPVL MKLLEEAGGN WSYIKLDNYT ALVDAIYSVE DENKQSEGSS NGNRGKNLKV IDSPATLKKT YETRSASSGS SIQVVQKQPQ LSNGDRKRKY KSRIADITKG SESVKIPLVD DVGSEAVPKF TYIPHNIVYQ SAYLHVSLAR ISDEDCCANC KGNCLSADFP CTCARETSGE YAYTKEGLLK EKFLDTCLKM KKEPDSFPKV YCKDCPLERD HDKGTYGKCD GHLIRKFIKE CWRKCGCDMQ CGNRVVQRGI RCQLQVYFTQ EGKGWGLRTL QDLPKGTFIC EYIGEILTNT ELYDRNVRSS SERHTYPVTL DADWGSEKDL KDEEALCLDA TICGNVARFI NHRCEDANMI DIPIEIETPD RHYYHIAFFT LRDVKAMDEL TWDYMIDFND KSHPVKAFRC CCGSESCRDR KIKGSQGKSI ERRKIVSAKK QQGSKEVSKK RK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Histone methyltransferase that converts monomethylated 'Lys-9' of histone H3 (H3K9me1) to dimethylated 'Lys-9' (H3K9me2) in the absence of bound ubiquitin, and to trimethylated 'Lys-9' (H3K9me3) in the presence of bound ubiquitin. Acts in a locus-specific manner and contributes to the transcriptional silencing of pseudogenes and transposons. H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression.
Gene References into Functions
  1. The N-terminal and C-terminal parts of S4WIYLD constitute a surface that interacts with charged residues close to the hydrophobic patch of ubiquitin. PMID: 24625295
  2. SUVR4 is involved in the epigenetic defense mechanism by trimethylating H3K9 to suppress potentially harmful transposon activity PMID: 21423664
  3. A novel histone lysine methyltransferase specificity was demonstrated for SUVR4, in that monomethylated histone H3K9 is its preferred substrate in vitro. PMID: 17020925
Subcellular Location
Nucleus. Chromosome.
Protein Families
Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family
Database Links

KEGG: ath:AT3G04380

STRING: 3702.AT3G04380.1

UniGene: At.27206

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