Recombinant Arabidopsis thaliana Homeobox protein knotted-1-like 1 (KNAT1)

Code CSB-YP344396DOA
MSDS
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Source Yeast
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Code CSB-EP344396DOA
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Source E.coli
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Code CSB-EP344396DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP344396DOA
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Source Baculovirus
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Code CSB-MP344396DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KNAT1
Uniprot No.
Alternative Names
KNAT1; BP; BP1; At4g08150; F9M13.2Homeobox protein knotted-1-like 1; Protein BREVIPEDICELLUS; Protein KNAT1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-398
Target Protein Sequence
MEEYQHDNST TPQRVSFLYS PISSSNKNDN TSDTNNNNNN NNSSNYGPGY NNTNNNNHHH QHMLFPHMSS LLPQTTENCF RSDHDQPNNN NNPSVKSEAS SSRINHYSML MRAIHNTQEA NNNNNDNVSD VEAMKAKIIA HPHYSTLLQA YLDCQKIGAP PDVVDRITAA RQDFEARQQR STPSVSASSR DPELDQFMEA YCDMLVKYRE ELTRPIQEAM EFIRRIESQL SMLCQSPIHI LNNPDGKSDN MGSSDEEQEN NSGGETELPE IDPRAEDREL KNHLLKKYSG YLSSLKQELS KKKKKGKLPK EARQKLLTWW ELHYKWPYPS ESEKVALAES TGLDQKQINN WFINQRKRHW KPSEDMQFMV MDGLQHPHHA ALYMDGHYMG DGPYRLGP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May play a role in meristem function, and may be involved in maintaining cells in an undifferentiated, meristematic state, and its expression disappears at the same time the shoot apex undergoes the transition from vegetative to reproductive development. Positive regulator of LATERAL ORGAN BOUNDARIES (LOB). Probably binds to the DNA sequence 5'-TGAC-3'. Able to traffic from the L1 to the L2/L3 layers of the meristem, presumably through plasmodesmata.
Gene References into Functions
  1. Arabidopsis plants harboring loss-of-function KNOX2 alleles exhibit impaired differentiation of all aerial organs and have highly complex leaves, phenocopying gain-of-function KNOX1 alleles. PMID: 25671434
  2. In the cambium, KNAT1 and STM, contrary to their function in the shoot apical meristem, promote cell differentiation through repression of BOP genes. PMID: 25371365
  3. Arabidopsis ASYMMETRIC LEAVES complex physically interacts with PRC2 and recruits this complex to the homeobox genes BREVIPEDICELLUS and KNAT2 to stably silence these stem cell regulators in differentiating leaves. [BP] PMID: 23468429
  4. KNAT1 negatively modulates root skewing, possibly by regulating auxin transport PMID: 23889705
  5. This function of STM in meristem establishment and organisation can be replaced by KNAT1/BP, but not KNAT2. PMID: 23573875
  6. The data indicate that the ATH1-KNAT2 complex acts redundantly with KNAT6, both of which are negatively regulated by BP during pedicel development. PMID: 22140242
  7. Results indicate that BP/KNAT1 acts as a negative regulator and KNAT2 and KNAT6 act as positive regulators of floral organ separation in the IDA-HAE/HSL2 signaling cascade. PMID: 21742991
  8. Results suggested that the late-flowering phenotype of the as1-1 and as2-1 mutants might have been caused by the ectopic expression of KNAT126 triple mutation. PMID: 19891706
  9. Heterodimers with PENNYWISE or POUND-FOOLISH are essential for establishing early internode patterning events. PMID: 16741748
  10. data showed that BP and PNY restrict KNAT6 and KNAT2 expression to promote correct inflorescence development PMID: 18390591
  11. Expression of KNAT1 in the integuments leads to extra cell divisions in the outer layer of the outer integument, and being compensated for by a decrease of cell volume, thus showing that mechanisms exist to control proper ovule integument morphogenesis. PMID: 18410683

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Subcellular Location
Nucleus.
Protein Families
TALE/KNOX homeobox family
Tissue Specificity
Expressed in the vegetative meristem. Present in the base of flower primordia.
Database Links

KEGG: ath:AT4G08150

STRING: 3702.AT4G08150.1

UniGene: At.28060

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