Recombinant Arabidopsis thaliana Hydroxyacylglutathione hydrolase 3, mitochondrial (GLY3)

Code CSB-YP861252DOA
MSDS
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Source Yeast
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Code CSB-EP861252DOA
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Source E.coli
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Code CSB-EP861252DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP861252DOA
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Source Baculovirus
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Code CSB-MP861252DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GLY3
Uniprot No.
Alternative Names
GLY3; At1g53580; F22G10.9; T3F20.11Persulfide dioxygenase ETHE1 homolog; mitochondrial; EC 1.13.11.18; Glyoxalase II; Glx II; Sulfur dioxygenase ETHE1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
53-294
Target Protein Sequence
LFRQLFEN ESSTFTYLLA DVSHPDKPAL LIDPVDKTVD RDLKLIDELG LKLIYAMNTH VHADHVTGTG LLKTKLPGVK SVISKASGSK ADLFLEPGDK VSIGDIYLEV RATPGHTAGC VTYVTGEGAD QPQPRMAFTG DAVLIRGCGR TDFQEGSSDQ LYESVHSQIF TLPKDTLIYP AHDYKGFEVS TVGEEMQHNP RLTKDKETFK TIMSNLNLSY PKMIDVAVPA NMVCGLQDVP SQAN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Sulfur dioxygenase that plays an essential role in hydrogen sulfide catabolism in the mitochondrial matrix. Hydrogen sulfide (H(2)S) gives rise to cysteine persulfide residues. ETHE1 consumes molecular oxygen to catalyze the oxidation of the persulfide, once it has been transferred to a thiophilic acceptor, such as glutathione (R-SSH). Plays an important role in metabolic homeostasis in mitochondria by metabolizing hydrogen sulfide and preventing the accumulation of supraphysiological H(2)S levels that have toxic effects, due to the inhibition of cytochrome c oxidase. Required for normal endosperm development in seed, and thereby also required for normal embryo development.
Gene References into Functions
  1. ETHE1 also affects the oxidation of branched-chain amino acids and lysine. PMID: 24692429
  2. ETHE1 is esential for early seed development. PMID: 22786886
  3. structural comparison of human ETHE1 and At1g53580 from Arabidopsis thaliana PMID: 16929096
Subcellular Location
Mitochondrion.
Protein Families
Metallo-beta-lactamase superfamily, Glyoxalase II family
Database Links

KEGG: ath:AT1G53580

STRING: 3702.AT1G53580.1

UniGene: At.23285

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