Recombinant Arabidopsis thaliana Hydroxymethylglutaryl-CoA synthase (HMGS)

Code CSB-YP010567DOA
MSDS
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Source Yeast
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Code CSB-EP010567DOA
MSDS
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Source E.coli
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Code CSB-EP010567DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP010567DOA
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Source Baculovirus
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Code CSB-MP010567DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HMGS
Uniprot No.
Alternative Names
HMGS; EMB2778; FKP1; MVA1; At4g11820; T26M18.30; Hydroxymethylglutaryl-CoA synthase; HMG-CoA synthase; EC 2.3.3.10; 3-hydroxy-3-methylglutaryl coenzyme A synthase; Protein EMBRYO DEFECTIVE 2778; Protein FLAKY POLLEN 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-461
Target Protein Sequence
MAKNVGILAM DIYFPPTCVQ QEALEAHDGA SKGKYTIGLG QDCLAFCTEL EDVISMSFNA VTSLFEKYKI DPNQIGRLEV GSETVIDKSK SIKTFLMQLF EKCGNTDVEG VDSTNACYGG TAALLNCVNW VESNSWDGRY GLVICTDSAV YAEGPARPTG GAAAIAMLIG PDAPIVFESK LRASHMAHVY DFYKPNLASE YPVVDGKLSQ TCYLMALDSC YKHLCNKFEK IEGKEFSIND ADYIVFHSPY NKLVQKSFAR LLYNDFLRNA SSIDEAAKEK FTPYSSLTLD ESYQSRDLEK VSQQIAKPFY DAKVQPTTLI PKEVGNMYTA SLYAAFASLI HKKHNDLAGK RVVMFSYGSG STATMFSLRL NDNKPPFSIS NIASVMDVGG KLKARHEYAP EKFVETMKLM EHRYGAKDFV TTKEGIIDLL APGTYYLKEV DSLYRRFYGK KGEDGSVANG H
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. Devoided of acetoacetyl-CoA thiolase (AACT) activity. Required for the development of both tapetosomes and elaioplasts in tapetal cells and for pollen viability during pollen tube elongation.
Gene References into Functions
  1. FLAKY POLLEN1 is required for organelle development in tapetal cells and pollen coat formation. [FKP1] PMID: 20484369
Protein Families
HMG-CoA synthase family
Tissue Specificity
Highly expressed in flower buds, pollen grains and anthers in the tapetal cells, at an intermediate levels in roots and weakly in leaves.
Database Links

KEGG: ath:AT4G11820

STRING: 3702.AT4G11820.2

UniGene: At.5362

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