Recombinant Arabidopsis thaliana IAA-amino acid hydrolase ILR1 (ILR1)

Code CSB-YP345031DOA
MSDS
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Source Yeast
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Code CSB-EP345031DOA
MSDS
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Source E.coli
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Code CSB-EP345031DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP345031DOA
MSDS
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Source Baculovirus
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Code CSB-MP345031DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ILR1
Uniprot No.
Alternative Names
ILR1; At3g02875; F13E7.18IAA-amino acid hydrolase ILR1; EC 3.5.1.-
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
24-442
Target Protein Sequence
GSYDSGS GLESLARGML HSAKDPEFFE WMRGIRRKIH ENPETGFQEF KTSQLVRDEL DSLGVKYKYP VAKTGVVAWI GSCSKPVFGL RADMDALPLQ ELVEWESKSK VDGKMHACGH DTHVAMLLGA AKLLQTTKHL IKGTVKLVFQ PGEEGYAGAY EMLKDEILDD LDGILSVHVF PSIPSGGIGS RPGTVLAGAG LFTVTVHGQG SHAATPHFSK DPVLAASSAV VALQQIVSRE LDPLEAGVVT VGYIEGGHAQ NVIPQSAKFG GTFRSLSNDG LLFIQRRIKE ISEAQASVYR CKAEVNFEEK KPSLHPVMNN DEGLYEHGKK VAEAMIGKNN FHDFPVTMGG EDFSFFTQKT KAAIFVLGVK NETLGAGKPL HSPYFFVDEE ALPVGAALHA AMAVSYLDEH GHSHEEEVKS EL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Hydrolyzes certain amino acid conjugates of the plant growth regulator indole-3-acetic acid (IAA), including IAA-Phe, IAA-Leu and IAA-Tyr. Can also use IAA-Ala, IAA-Gly, IAA-Met and IAA-Glu as substrates with low efficiency. No activity with IAA-Ile, IAA-1-O-beta-D-glucose or IAA-myo-inositol. Is the most efficient enzyme of the ILL family for IAA-Leu hydrolysis. Important for IAA-Leu and IAA-Phe hydrolysis in roots. May act with ILL2 to provide free IAA to germinating seedlings.
Protein Families
Peptidase M20 family
Tissue Specificity
Expressed in leaves, stems, siliques, seeds and flowers. Detected in central regions of cotyledons, hypocotyl, radicle of mature embryos and seedlings, micropyle of siliques and in pollen.
Database Links

KEGG: ath:AT3G02875

STRING: 3702.AT3G02875.1

UniGene: At.18770

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