Recombinant Arabidopsis thaliana IAA-amino acid hydrolase ILR1-like 6 (ILL6)

Code CSB-YP851893DOA
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Source Yeast
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Code CSB-EP851893DOA
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Source E.coli
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Code CSB-EP851893DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP851893DOA
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Source Baculovirus
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Code CSB-MP851893DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ILL6
Uniprot No.
Alternative Names
ILL6; GR1; At1g44350; T18F15.9; IAA-amino acid hydrolase ILR1-like 6; EC 3.5.1.-; Protein gr1; jasmonoyl-L-amino acid hydrolase; EC 3.5.1.127
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
25-464
Target Protein Sequence
TNLPFF EVKYPNNNPF GMLLRPTPIK NQSLGLPAHV GSDECRVWTK ACSDEILRLT YQPDNVAWLK RVRRTIHENP ELAFEEYETS RLIRSELDRM GIMYRYPLAK TGIRAWIGSG GPPFVAVRAD MDALPIQEAV EWEHISKVAG KMHACGHDAH VTMLLGAAHI LKAREHLLKG TVVLLFQPAE EAGNGAKNMI EDGALDDVEA IFAVHVSHIH PTGVIGSRSG PLLAGCGIFR AVITSEDSRG AANLLLAASS AVISLQGIVS REASPLDSQV VSVTSFDGGH SLDVAPDTVV LGGTFRAFSN SSFYYLKKRI QEVLMDQVGV FGCQATVNFF EKQNAIYPPT TNNDATYNHL KKVTIDLLGD SHFTLAPQMM GAEDFAFYSE IIPAAFYFIG IRNEELGSVH IAHSPHFMID EDSLPVGAAV HAAVAERYLN DKHS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Hydrolyzes certain amino acid conjugates of the plant growth regulator indole-3-acetic acid (IAA). Also hydrolyzes amino acid conjugates of jasmonic acid and 12-hydroxy jasmonic acid.
Gene References into Functions
  1. Plants carrying ILL6 loss-of-function mutations have reduced ability to hydrolyze exogenous or endogenous JA-Ile. PMID: 23943861
  2. The amidohydrolases IAR3 and ILL6 contribute to jasmonoyl-isoleucine hormone turnover and generate 12-hydroxyjasmonic acid upon wounding in Arabidopsis leaves. PMID: 24052260
Protein Families
Peptidase M20 family
Database Links

KEGG: ath:AT1G44350

STRING: 3702.AT1G44350.1

UniGene: At.28163

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