Recombinant Arabidopsis thaliana Inactive poly [ADP-ribose] polymerase RCD1 (RCD1), partial

Code CSB-YP840435DOA
MSDS
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Source Yeast
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Code CSB-EP840435DOA
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Source E.coli
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Code CSB-EP840435DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP840435DOA
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Source Baculovirus
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Code CSB-MP840435DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RCD1
Uniprot No.
Alternative Names
RCD1; ATP8; CEO1; At1g32230; F3C3.1; Inactive poly [ADP-ribose] polymerase RCD1; Protein RADICAL-INDUCED CELL DEATH 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Inactive ADP-ribosyltransferase that functions with SRO1 to regulate oxidative stress, hormonal and developmental responses. Required for embryogenesis, vegetative and reproductive development, and abiotic stress responses. May regulate several stress-responsive genes. Seems to play a larger developmental role than SRO1. Does not bind NAD in vitro.
Gene References into Functions
  1. not directly involved in the solar UV-B regulation of genes with functions in UV acclimation, hormone signalling and stress-related markers PMID: 24689869
  2. Using a rcd1 double mutant collection we show that SIMR is mostly independent of the classical plant defense signaling pathways and that the redox balance is involved in development of SIMR. PMID: 24550736
  3. The radical-induced cell death1 (rcd1) mutant defective in responses to reactive oxygen species (ROS) was isolated as an enhancer of the snc1 mutant in growth inhibition but not in defense response activation. PMID: 23365132
  4. RCD1 is rapidly degraded during heat stress, thus our results suggest that removal of RCD1 protein or the loss of the interaction domain in DREB2A appears to be required for proper DREB2A function under stress conditions. PMID: 22150398
  5. We examined O(3) and superoxide-induced cell death in the O(3)-sensitive radical-induced cell death1 (rcd1) mutant. PMID: 15728341
  6. The overexpression of rcd1-1 cDNA has been shown to bring about an rcd1-like phenotype, we created and examined the overexpression lines of RCD1 by the use of the cauliflower mosaic virus 35S promoter. PMID: 16926493
  7. RCD1, working together with STO, is involved in Arabidopsis UV-B signaling PMID: 19492112
  8. RCD1 interacts with several transcription factors which are involved in both developmental and stress-related processes. PMID: 19548978

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Subcellular Location
Nucleus matrix. Note=Speckle-like pattern.
Tissue Specificity
Expressed in young developing tissues, such as young leaves and flowers and root tips. In mature plants, expressed in vasculature of leaves and roots, and guard cells.
Database Links

KEGG: ath:AT1G32230

STRING: 3702.AT1G32230.1

UniGene: At.10171

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