Recombinant Arabidopsis thaliana Indole-3-acetic acid-amido synthetase GH3.5 (GH3.5), partial

Code CSB-YP526844DOA
MSDS
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Source Yeast
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Code CSB-EP526844DOA
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Source E.coli
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Code CSB-EP526844DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP526844DOA
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Source Baculovirus
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Code CSB-MP526844DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GH3.5
Uniprot No.
Alternative Names
GH3.5; At4g27260; M4I22.70Indole-3-acetic acid-amido synthetase GH3.5; EC 6.3.2.-; Auxin-responsive GH3-like protein 5; AtGH3-5
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the synthesis of indole-3-acetic acid (IAA)-amino acid conjugates, providing a mechanism for the plant to cope with the presence of excess auxin. Strongly reactive with Glu, Gln, Trp, Asp, Ala, Leu, Phe, Gly, Tyr, Met, Ile and Val. Little or no product formation with His, Ser, Thr, Arg, Lys, or Cys. Also active on pyruvic and butyric acid analogs of IAA, PAA and the synthetic auxin naphthaleneacetic acid (NAA). The two chlorinated synthetic auxin herbicides 2,4-D and 3,6-dichloro-o-anisic acid (dicamba) cannot be used as substrates.
Gene References into Functions
  1. This study suggests that AtGH3.5 conjugates auxins (i.e., IAA and PAA) and benzoates (i.e., SA and BA) to mediate crosstalk between different metabolic pathways, broadening the potential roles for GH3 acyl acid amido synthetases in plants. PMID: 27849615
  2. Arabidopsis acetyl-amido synthetase GH3.5 involvement in camalexin biosynthesis through conjugation of indole-3-carboxylic acid and cysteine and upregulation of camalexin biosynthesis genes. PMID: 22624950
  3. GH3.5 gene is required for fine-tuning adventitious root initiation in the Arabidopsis thaliana hypocotyl, and is acting by modulating jasmonic acid homeostasis PMID: 22730403
  4. Results provide support for WES1 regulating hypocotyl growth by mediating phytochrome B-perceived light signals. PMID: 17602188
  5. This study shows that GH3.5 acts as a bifunctional modulator in both salicylic acid and auxin signaling during pathogen infection. PMID: 17704230

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Protein Families
IAA-amido conjugating enzyme family
Database Links

KEGG: ath:AT4G27260

STRING: 3702.AT4G27260.1

UniGene: At.2338

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