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Recombinant Arabidopsis thaliana Inositol polyphosphate multikinase alpha (IPK2a)

Recombinant Arabidopsis thaliana Inositol polyphosphate multikinase alpha (IPK2a)

Code	CSB-YP881858DOA
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP881858DOA
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP881858DOA-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP881858DOA
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP881858DOA
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

IPK2a

Uniprot No.

Q9LY23

Alternative Names

IPK2a; IP3K; IPMK; At5g07370; T2I1.80Inositol polyphosphate multikinase alpha; EC 2.7.1.140; EC 2.7.1.151; Inositol polyphosphate 6-/3-/5-kinase alpha; AtIpk2-alpha; AtIpk2alpha

Species

Arabidopsis thaliana (Mouse-ear cress)

Expression Region

1-286

Target Protein Sequence

MQLKVPEHQV AGHIAKDGKP GPLVDDKGRF FKPLQGDSRG EIEVKFYESF SSNTEVPEHI HRYFPVYHGT QAVEGSDGAA MMVLENLLAE YTKPSVMDVK MGSRTWYPDA SEEYIQKCLK KDTGTTTVSS GFRISGFEVY DHKESSFWKP ERKLLRGLDV DGARLTLRKF VSSNSLSDTG SKPDSAFASS VYGGSHGILT QLLELKTWFE NQTLYHFNSC SILMVYENES ILKGNDDDAR PQVKLVDFAH VLDGNGVIDH NFLGGLCSFI NFIREILQSP DESADS

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Inositol phosphate kinase with a broad substrate specificity. Phosphorylates inositol 1,4,5-trisphosphate (Ins(1,4,5)P3), inositol 1,4,5,6-tetrakisphosphate (Ins(1,4,5,6)P4), inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4), inositol 1,3,4,6-tetrakisphosphate (Ins(1,3,4,6)P4) and inositol 1,2,3,4,6-pentakisphosphate (Ins(1,2,3,4,6)P5) but not inositol 1,4-bisphosphate (Ins(1,4)P2), inositol 1,3,4-trisphosphate (Ins(1,3,4)P3), inositol 1,2,6-trisphosphate (Ins(1,2,6)P3), inositol 3,4,5,6-tetrakisphosphate (Ins(3,4,5,6)P4), inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5), inositol 1,2,4,5,6-pentakisphosphate (Ins(1,2,4,5,6)P5) or inositol hexakisphosphate (InsP6). Regulates pollen and root development probably through the regulation of InsP3-mediated calcium accumulation.

Gene References into Functions

AtIPK2alpha and AtIPK2beta are involved in pollen development, pollen tube guidance and embryogenesis. PMID: 25846941
The physiological function of an A. thaliana gene encoding inositol polyphosphate kinase in pollen germination and root growth.[AtIPK2α] PMID: 15618435

Subcellular Location

Nucleus. Cell membrane.

Protein Families

Inositol phosphokinase (IPK) family

Tissue Specificity

Detected in leaves, stems, roots, siliques and flowers. Highly expressed in root tissues, anthers, the stigma, pollen grains and growing pollen tubes.

Database Links

KEGG: ath:AT5G07370

STRING: 3702.AT5G07370.1

UniGene: At.24944

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