Recombinant Arabidopsis thaliana Inositol polyphosphate multikinase beta (IPK2b)

Code CSB-YP884290DOA
MSDS
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Source Yeast
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Code CSB-EP884290DOA
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Source E.coli
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Code CSB-EP884290DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP884290DOA
MSDS
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Source Baculovirus
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Code CSB-MP884290DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
IPK2b
Uniprot No.
Alternative Names
IPK2b; IP3K; IPMK; At5g61760; MAC9.7; MAC9_60Inositol polyphosphate multikinase beta; EC 2.7.1.140; EC 2.7.1.151; Inositol polyphosphate 6-/3-/5-kinase beta; AtIpk2-beta; AtIpk2beta
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-300
Target Protein Sequence
MLKVPEHQVA GHIASDGKLG PLVDDQGRFF KPLQGDSRGE HEAKFYESFT SNMKVPDHIH RYFPVYHGTQ LVEASDGSGK LPHLVLDDVV SGYANPSVMD VKIGSRTWYP DVSEEYFKKC IKKDRQTTTV SLGFRVSGFK IFDHQESSFW RAEKKLVLGY NADGARLALR KFVSSNSPAD SNLTPNCAFA SEVYGGCNGI LAQLLELKDW FETQTLYHFN SCSILMIYEN ESILMQGGDD APAPRAQVKL VDFAHVLDGN GVIDHNFLGG LCSFIKFIKD ILQSVEKHDE TDTSLLENGR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Inositol phosphate kinase with a broad substrate specificity. Phosphorylates inositol 1,4,5-trisphosphate (Ins(1,4,5)P3), inositol 1,4,5,6-tetrakisphosphate (Ins(1,4,5,6)P4), inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4), inositol 1,3,4,6-tetrakisphosphate (Ins(1,3,4,6)P4) and inositol 1,2,3,4,6-pentakisphosphate (Ins(1,2,3,4,6)P5) but not inositol 1,4-bisphosphate (Ins(1,4)P2), inositol 1,3,4-trisphosphate (Ins(1,3,4)P3), inositol 1,2,6-trisphosphate (Ins(1,2,6)P3), inositol 3,4,5,6-tetrakisphosphate (Ins(3,4,5,6)P4), inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5), inositol 1,2,4,5,6-pentakisphosphate (Ins(1,2,4,5,6)P5) or inositol hexakisphosphate (InsP6). Involved in the auxin signaling pathway. Regulates axillary shoot branching and is required for phytate synthesis in seeds.
Gene References into Functions
  1. AtIPK2beta and IP6 are involved in glucose suppression of seed germination and that AtIPK2beta enzyme activity is likely to be regulated by SnRK1.1. PMID: 29216370
  2. for AtIpk2beta in axillary shoot branching through the auxin signaling pathway PMID: 17434984
Subcellular Location
Nucleus.
Protein Families
Inositol phosphokinase (IPK) family
Tissue Specificity
Expressed in leaves, stems, roots, siliques and flowers. Detected in vascular strands, stigma cells, the abscission zones of fully elongated siliques, the root central cylinder and the root tip.
Database Links

KEGG: ath:AT5G61760

STRING: 3702.AT5G61760.1

UniGene: At.8495

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