Recombinant Arabidopsis thaliana Interactor of constitutive active ROPs 5 (ICR5)

Code CSB-YP837779DOA
MSDS
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Source Yeast
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Code CSB-EP837779DOA
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Source E.coli
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Code CSB-EP837779DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP837779DOA
MSDS
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Source Baculovirus
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Code CSB-MP837779DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ICR5
Uniprot No.
Alternative Names
ICR5; MIDD1; RIP3; At3g53350; F4P12.50; Interactor of constitutive active ROPs 5; Protein MICROTUBULE DEPLETION DOMAIN 1; ROP-interactive partner 3
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-396
Target Protein Sequence
MQTPKSRPGS LELPQKKSPL PAPKVVRRLK PSGAESDPKT KTISKTQIPK VVADRRSARI PLNEIQKKRT GRIPELESTI SQLQEELKKA KEELNRSEAL KREAQEEAED AKHQLMDINA SEDSRIEELR KLSQERDKTW QSELEAMQRQ HGMDSTALSS AINEVQKLKS KLFESESELE QSKYEVRSLE KLVRQLEEER VNSRDSSSSM EVEELKEAMN LSRQEITQLK SAVEAAETRY QEEYIQSTLQ IRSAYEQTEA VKSRYSQREA ELTEELNRTK DEIEGLRKEL MEKVKEDEST GDLKKLESDL MEVRGSLMDK EMELQILRSA MEKKVETANT EAMEAELKRV KIQCEQWRKA AETAASILNN DEERTDSIET SKMLKKFGVL LKKNHK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
ROP effector binding specifically activated ROPs and linking them to the microtubule cytoskeleton. Involved in ROP-regulated polar growth. Involved in local disassembly of cortical microtubules when associated with ARAC10 and KIN13A and conversely mediates also the elimination of ARAC10 from the plasma membrane by the cortical microtubules. Accumulates at the plus end of shrinking microtubules. Targets KIN13A to microtubules.
Gene References into Functions
  1. Data show that RIP3 is localized at microtubules and interacts with the kinesin-13 family member AtKinesin-13A, suggesting a role for RIP3 in microtubule reorganization and a possible function in ROP-regulated polar growth. PMID: 20832900
Subcellular Location
Cell membrane. Cytoplasm, cytoskeleton.
Protein Families
ICR family
Tissue Specificity
Expressed in xylem cells in the roots and in stamens, petals and pollen.
Database Links

KEGG: ath:AT3G53350

STRING: 3702.AT3G53350.1

UniGene: At.28433

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