Recombinant Arabidopsis thaliana Isochorismate synthase 1, chloroplastic (ICS1), partial

Code CSB-YP874349DOA
MSDS
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Source Yeast
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Code CSB-EP874349DOA
MSDS
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Source E.coli
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Code CSB-EP874349DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP874349DOA
MSDS
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Source Baculovirus
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Code CSB-MP874349DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ICS1
Uniprot No.
Alternative Names
ICS1; At1g74710; F1M20.39; F25A4.31Isochorismate synthase 1; chloroplastic; AtIcs1; IcsI; EC 5.4.4.2; Enhanced disease susceptibility 16; Eds16; Isochorismate mutase 1; Salicylic acid induction deficient 2; Sid2; menF-like protein 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the synthesis of salicylic acid (SA) required for both local and systemic acquired resistance (LAR and SAR) while SA synthesized through the phenylalanine ammonium lyase (PAL) pathway seems to potentiate plant cell death. Also involved in phylloquinone (vitamin K1) synthesis. Has no isochorismate pyruvate lyase (IPL) activity.
Gene References into Functions
  1. Photosynthetic parameters in the ics1 mutant changed in a manner observed previously for state transition-impaired plants. Deregulation of the redox status of the plastoquinone pool in ics1 showed significant variation depending on the leaf age. PMID: 23956412
  2. Lesions driven by intracellular H2O2 require SA synthesis through the isochorismate pathway. PMID: 20543092
  3. sid2 gene, which encodes for isochorismate synthase, plays a central role in SA biosynthesis during plant development not only in leaves, but also in seeds of A. thaliana. PMID: 19188277
  4. Mutations in sid2 abolished the enhanced resistance of dnd mutants against Pseudomonas syringae pv. tomato and Hyaloperonospora parasitica but not Botrytis cinerea. PMID: 18785824
  5. SID2-independent defenses are activated in acd6-1 mutants, and accumulation of camalexin is largely independent of SID2. PMID: 19144005
  6. Salicylic acid-deficient NahG transgenic lines and sid2 mutants were used to evaluate the role of this compound in the development of the short-lived, annual plant Arabidopsis thaliana. PMID: 19188277

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Subcellular Location
Plastid, chloroplast.
Protein Families
Isochorismate synthase family
Tissue Specificity
Leaves.
Database Links

KEGG: ath:AT1G74710

STRING: 3702.AT1G74710.2

UniGene: At.22628

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