Recombinant Arabidopsis thaliana Isovaleryl-CoA dehydrogenase, mitochondrial (IVD)

Code CSB-YP886803DOA
MSDS
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Source Yeast
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Code CSB-EP886803DOA
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Source E.coli
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Code CSB-EP886803DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP886803DOA
MSDS
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Source Baculovirus
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Code CSB-MP886803DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
IVD
Uniprot No.
Alternative Names
IVD; At3g45300; F18N11.6; Isovaleryl-CoA dehydrogenase; mitochondrial; IVD; EC 1.3.8.4
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
26-409
Target Protein Sequence
SSSLL FDDTQLQFKE SVSKFAQDNI APHAERIDKT NSFPKDVNLW KLMGEFNLHG ITAPEEYGGL GLGYLYHCIA MEEISRASGS VALSYGAHSN LCINQLVRNG TAAQKEKYLP KLISGEHVGA LAMSEPNAGS DVVGMKCKAE KVDGGYILNG NKMWCTNGPS AETLVVYAKT DTKAGSKGIT AFIIEKGMTG FSTAQKLDKL GMRGSDTCEL VFENCFVPEE NILDKEGKGV YVLMSGLDLE RLVLAAGPLG IMQACLDNVL PYIRQREQFG RPVGEFQFIQ GKVADMYTAL QSSRSYVYSV ARDCDNGKVD PKDCAGTILC AAERATQVAL QAIQCLGGNG YINEYATGRL LRDAKLYEIG AGTSEIRRIV IGRELFKEE
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in degradation of the branched-chain amino acids, phytol and lysine for the supply of carbon and electrons to the ETF/ETFQO complex during dark-induced sugar starvation.
Gene References into Functions
  1. Data elucidates the pathway of plant Lys catabolism and demonstrate that both isovaleryl-CoA dehydrogenase and 2-hydroxyglutarate dehydrogenase act as electron donors to the ubiquinol pool via an ETF/ETFQO-mediated route. [IVDH] PMID: 20501910
  2. Accumulation of both homomethionine and 3-isovaleroyloxypropyl-glucosinolate cosegregates with the high branched chain amino acid phenotype in IVD mutants. PMID: 19929878
Subcellular Location
Mitochondrion.
Protein Families
Acyl-CoA dehydrogenase family
Tissue Specificity
Expressed in leaves, stems and flowers. Not detected in roots.
Database Links

KEGG: ath:AT3G45300

STRING: 3702.AT3G45300.1

UniGene: At.428

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