Recombinant Arabidopsis thaliana LOB domain-containing protein 30 (LBD30)

Code CSB-YP528063DOA
MSDS
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Source Yeast
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Code CSB-EP528063DOA
MSDS
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Source E.coli
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Code CSB-EP528063DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP528063DOA
MSDS
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Source Baculovirus
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Code CSB-MP528063DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LBD30
Uniprot No.
Alternative Names
LBD30; ASL19; At4g00220; F6N15.4; LOB domain-containing protein 30; ASYMMETRIC LEAVES 2-like protein 19; AS2-like protein 19
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-228
Target Protein Sequence
MSSSGNPSSS SGGGGGPCGA CKFLRRKCVA GCIFAPYFDS EQGAAHFAAV HKVFGASNVS KLLHHVPEHK RPDAVVSICF EAQARLRDPI YGCVSHIVSL QQQVVSLQTE LSYLQAHLAT LELPQPQPPQ VPVSSSGSLQ ALSITDLPTI SPSVYDLSSI FEPVMSSTWA MQQQPRPSDH LFGVPSSSNM GGGGELQALA REFIHGGQMP AQPSPGTSGS ASSVIKRE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the positive regulation of tracheary element (TE) differentiation. Involved in a positive feedback loop that maintains or promotes NAC030/VND7 expression that regulates TE differentiation-related genes.
Gene References into Functions
  1. JLO can regulate auxin perception and consequently auxin transport and auxin-responsive gene expression PMID: 28472464
  2. JLO is a central regulator of auxin distribution and signaling throughout plant development. PMID: 20852917
  3. Jagged Lateral Organs (JLO) acts from the boundary to orchestrate the drastic changes in gene expression that entail the initiation of plant lateral organs. [JLO] PMID: 17557810
  4. ASL19 were expressed in immature tracheary elements (TEs), and the expression was dependent on VASCULAR-RELATED NAC-DOMAIN PROTEIN6 (VND6) and VND7, which are transcription factors required for TE differentiation. PMID: 19088331
Protein Families
LOB domain-containing protein family
Tissue Specificity
Expressed in roots, stems, leaves and flowers. Expressed in vascular tissues of hypocotyls, leaves, roots, developing floral organs and siliques.
Database Links

KEGG: ath:AT4G00220

STRING: 3702.AT4G00220.1

UniGene: At.21182

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