Recombinant Arabidopsis thaliana MADS-box protein SOC1 (SOC1)

Code CSB-YP525357DOA
MSDS
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Source Yeast
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Code CSB-EP525357DOA
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Source E.coli
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Code CSB-EP525357DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP525357DOA
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Source Baculovirus
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Code CSB-MP525357DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SOC1
Uniprot No.
Alternative Names
SOC1; AGL20; At2g45660; F17K2.19; MADS-box protein SOC1; Agamous-like MADS-box protein AGL20; Protein SUPPRESSOR OF CONSTANS OVEREXPRESSION 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-214
Target Protein Sequence
MVRGKTQMKR IENATSRQVT FSKRRNGLLK KAFELSVLCD AEVSLIIFSP KGKLYEFASS NMQDTIDRYL RHTKDRVSTK PVSEENMQHL KYEAANMMKK IEQLEASKRK LLGEGIGTCS IEELQQIEQQ LEKSVKCIRA RKTQVFKEQI EQLKQKEKAL AAENEKLSEK WGSHESEVWS NKNQESTGRG DEESSPSSEV ETQLFIGLPC SSRK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription activator active in flowering time control. May integrate signals from the photoperiod, vernalization and autonomous floral induction pathways. Can modulate class B and C homeotic genes expression. When associated with AGL24, mediates effect of gibberellins on flowering under short-day conditions, and regulates the expression of LEAFY (LFY), which links floral induction and floral development.
Gene References into Functions
  1. Results indicate that SUPPRESSOR OF OVEREXPRESSION OF CO 1 (SOC1) inhibits chlorophyll degradation via negatively regulating pheophytinase (PPH) expression. PMID: 28096189
  2. We also demonstrate that a domain of the C-terminal region of ESD7 mediates the binding to the different PRC2 components and this interaction is necessary for the proper recruitment of PRC2 to FT and SOC1 chromatin. PMID: 26980282
  3. SPL15 and the MADS-box protein SOC1 cooperate to promote transcription of their target genes. PMID: 27134142
  4. SOC1 is involved in the regulation of stomatal opening via transcriptional regulation in guard cells. PMID: 25588388
  5. a cross-repressive interaction of GNC and GNL on the one side and SOC1 on the other in the control of flowering time, greening, and cold tolerance PMID: 23739688
  6. The drought escape response requires GI, FT/TSF, and AGL20 proteins in Arabidopsis.Abscisic acid up-regulates AGL20 expression in a photoperiod-dependent manner. PMID: 23719890
  7. 3' UTR-mediated SOC1 mRNA destabilization by AtBRN1 and AtBRN2 proteins. PMID: 23437850
  8. Our studies provide insight into the role of MPF2-like genes in phase transition by interacting with SOC1 and MAF1 genes, thereby also pointing to their significance as potential candidates for modifying flowering in crop plants in the future. PMID: 22539207
  9. Flowering is controlled by AGL24 partly independently of SOC1 and FUL. PMID: 22902690
  10. Flowering was promoted by induction of FT and SOC1 expression with blue light in Arabidopsis, whereas GpFTs and GpSOC1 expression was low with blue light induction in G. paniculata. PMID: 21431295
  11. SOC1 constitutes a major hub in the regulatory networks underlying floral timing and flower development and that these networks are composed of many positive and negative autoregulatory and feedback loops. PMID: 22791302
  12. SOC1 directly controls the expression of AGL42, AGL71 and AGL72 to balance the expression level of these SOC1-like genes. PMID: 21609362
  13. Results demonstrate the presence of feedback loop that delays flowering through the increase of FLC when a cold spell is transient but suppresses the cold response when floral induction occurs through the repression of cold-inducible genes by SOC1. PMID: 19825833
  14. SOC1 is able to bind directly to regulatory regions of class B and C homeotic genes. PMID: 19656343
  15. Activation of SOC1 expression by FT and TSF in winter-annual flowering A. thaliana. [SOC1] PMID: 15618421
  16. FT regulates SOC1 expression, and SOC1 regulates LFY expression [SOC1] PMID: 15695467
  17. inactivation of FT caused down-regulation of SOC1 even in plants overexpressing CO, indicating that FT is required for SOC1 induction by CO PMID: 16183837
  18. SOC1 gene. MADS box proteins are thought to bind their DNA targets as dimers or higher-order multimers PMID: 16623882
  19. during floral transition, a positive-feedback loop conferred by direct transcriptional regulation between AGL24 and SOC1 at the shoot apex integrates flowering signals PMID: 18339670
  20. Interaction with AGL24 relocates SOC1 to the nucleus, where SOC1 regulates leafy expression by binding to the LFY promoter. PMID: 18466303
  21. EARLY FLOWERING9 (ELF9), an Arabidopsis thaliana RNA binding protein, directly targets the SOC1 transcript and reduces SOC1 mRNA levels. PMID: 19376936

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Subcellular Location
Nucleus. Cytoplasm. Note=Translocation from the cytoplasm to the nucleus in the presence of AGL24.
Tissue Specificity
Widely expressed. Not found in the apical meristem of short-day grown plants in vegetative stage.
Database Links

KEGG: ath:AT2G45660

STRING: 3702.AT2G45660.1

UniGene: At.25546

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