Recombinant Arabidopsis thaliana MIP18 family protein At1g68310 (At1g68310)

Code CSB-YP866402DOA
MSDS
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Source Yeast
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Code CSB-EP866402DOA
MSDS
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Source E.coli
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Code CSB-EP866402DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP866402DOA
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Source Baculovirus
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Code CSB-MP866402DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AE7
Uniprot No.
Alternative Names
AE7; At1g68310; T22E19.6Protein AE7; AS1/2 enhancer 7; Asymmetric leaves 1/2 enhancer 7
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-157
Target Protein Sequence
MVSGLINENP IIYPKKERRL RTDTSITDEL TPEPIDQLEI FDHIRDIKDP EHPNTLEDLR VVTEDSVEVD DENSYVRVTF TPTVEHCSMA TVIGLCVRVK LLRSLPSRYK IDIRVAPGSH ATEDALNKQL NDKERVAAAL ENPNLVEMVD ECLPSEE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Central member of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) pathway. Involved in leaf polarity formation. Promotes leaf adaxial identity. May play a role in the cell cycle progression and is required for cell proliferation.
Gene References into Functions
  1. AE7 is a central member of the cluster assembly pathway, linking plant mitochondria to nuclear genome integrity through assembly of Fe-S proteins. PMID: 23104832
  2. Study characterized and cloned an Arabidopsis gene, AS1/2 ENHANCER7 (AE7), which is required for both leaf adaxial-abaxial polarity formation and normal cell proliferation. [AE7] PMID: 21070412
Subcellular Location
Nucleus. Cytoplasm.
Protein Families
MIP18 family
Tissue Specificity
Expressed in the embryo, shoot apical meristem, leaf primordia, inflorescence and all floral organs.
Database Links

KEGG: ath:AT1G68310

STRING: 3702.AT1G68310.1

UniGene: At.35543

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