Recombinant Arabidopsis thaliana Metacaspase-5 (AMC5)

Code CSB-YP527702DOA
MSDS
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Source Yeast
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Code CSB-EP527702DOA
MSDS
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Source E.coli
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Code CSB-EP527702DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP527702DOA
MSDS
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Source Baculovirus
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Code CSB-MP527702DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AMC5
Uniprot No.
Alternative Names
AMC5; AMC6; MCP2B; At1g79330; YUP8H12R.5Metacaspase-5; AtMC5; EC 3.4.22.-; Metacaspase 2b; AtMCP2b; Metacaspase-6
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-410
Target Protein Sequence
MAKKAVLIGI NYPGTKAELR GCVNDVRRVH KSLVDRFGFS ERNITELIDT DESSTKPTGK NIRRALLNLV ESAKPGDVLV VHYSGHGTRL PAETGEDDDT GYDECIVPCD MNLITDDEFR DLVEKVPKEA HITIISDSCH SGGLIDEAKE QIGESTKKKP KKESGGSSGL GIKGFVREAV EEALESKGIA IPHHKDEKDE NKTKELKLED GAKVHVVNKS LPLQTLIDIL KQNTGNNDIE VGKIRPTLFN VFGEDASPKV KKFMKVILTK LQEGKTEGGI LGMIGKLAQE FLKHKLNDDE EYVKPAMKTH VGNKQEVYAG ASNGSLADNG ILISGCQTDQ TSADASPQGH PEMAYGAFTN AVQIILEETK GMITYKELVL KARKLLKKQG FSQRPGLYCS DSFVNAPFIC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Cysteine protease that cleaves specifically after arginine or lysine residues. Does not cleave caspase-specific substrates. May be involved in the modulation of programmed cell death activated by oxidative stress.
Gene References into Functions
  1. AtMCP1b and AtMCP2b are arginine/lysine-specific cysteine proteases with roles in apoptosis. [AtMCP2b] PMID: 15691845
Protein Families
Peptidase C14B family
Tissue Specificity
Expressed in roots, leaves, cauline leaves and flower buds.
Database Links

KEGG: ath:AT1G79330

STRING: 3702.AT1G79330.1

UniGene: At.34135

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