Recombinant Arabidopsis thaliana Methylthioalkylmalate synthase 3, chloroplastic (MAM3)

Code CSB-YP880805DOA
MSDS
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Source Yeast
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Code CSB-EP880805DOA
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Source E.coli
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Code CSB-EP880805DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP880805DOA
MSDS
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Source Baculovirus
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Code CSB-MP880805DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MAM3
Uniprot No.
Alternative Names
MAM3; IMS2; IPMS_AT1; MAM-L; MAM1; At5g23020; MYJ24.1; Methylthioalkylmalate synthase 3; chloroplastic; EC 2.3.3.17; 2-isopropylmalate synthase 2; Methylthioalkylmalate synthase-like
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
52-503
Target Protein Sequence
AESKKVATS ATDLKPIMER RPEYIPNKLP HKNYVRVLDT TLRDGEQSPG AALTPPQKLE IARQLAKLRV DIMEVGFPVS SEEEFEAIKT IAKTVGNEVD EETGYVPVIC GIARCKKRDI EATWEALKYA KRPRVMLFTS TSEIHMKYKL KKTKEEVIEM AVNSVKYAKS LGFKDIQFGC EDGGRTEKDF ICKILGESIK AGATTVGFAD TVGINMPQEF GELVAYVIEN TPGADDIVFA IHCHNDLGVA TANTISGICA GARQVEVTIN GIGERSGNAP LEEVVMALKC RGESLMDGVY TKIDSRQIMA TSKMVQEHTG MYVQPHKPIV GDNCFVHESG IHQDGILKNR STYEILSPED VGIVKSENSG IVLGKLSGRH AVKDRLKELG YEISDEKFND IFSRYRELTK DKKRITDADL KALVVNGAEI SSEKLNSKGI NDLMSSPQIS AVV
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Determines the side chain length of aliphatic glucosinolate structures. Accepts all the omega-methylthio-2-oxoalkanoic acids needed to form the known C3 to C8 glucosinolates. Also able to convert pyruvate to citramalate, 2-oxoisovalerate to isopropylmalate, 4-methyl-2-oxopentanoate and 5-methyl-2-oxohexanoate for Leu-derived glucosinolates, 3-methyl-2-oxopentanoate for Ile-derived glucosinolates and phenylpyruvate to phenylethylglucosinolate.
Gene References into Functions
  1. MAM3 (formerly known as MAM-L)catalyzes the formation of all glucosinolate chain lengths in vivo as well as in vitro. PMID: 17369439
Subcellular Location
Plastid, chloroplast.
Protein Families
Alpha-IPM synthase/homocitrate synthase family
Tissue Specificity
Highly expressed in roots, leaves, and siliques. Lower amounts in stems and flowers.
Database Links

KEGG: ath:AT5G23020

STRING: 3702.AT5G23020.1

UniGene: At.20469

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