Recombinant Arabidopsis thaliana Mitotic-spindle organizing protein 1A (At1g73790)

Code CSB-YP871699DOA
MSDS
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Source Yeast
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Code CSB-EP871699DOA
MSDS
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Source E.coli
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Code CSB-EP871699DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP871699DOA
MSDS
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Source Baculovirus
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Code CSB-MP871699DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GIP2
Uniprot No.
Alternative Names
GIP2; GIP1A; At1g73790; F25P22.21; Mitotic-spindle organizing protein 1A; GCP3-interacting protein 2; AtGIP2; Mitotic-spindle organizing protein associated with a ring of gamma-tubulin 1A; AtGIP1A
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-67
Target Protein Sequence
MNQEAAETAR ESLELVFRMS NILETGLDRH TLSVLIALCD IGLNPEALAT LVKELRRDSA TTTTTVD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for gamma-tubulin complex recruitment to the microtubule organizing centers (MTOCs). During mitosis, modulates gamma-tubulin complex localization, spindle stability and chromosomal segregation. Necessary for gametophyte development and embryogenesis.
Gene References into Functions
  1. GIPs are essential for the proper recruitment and/or stabilization of centromeric proteins essential in the specification of the centromere identity, as well as for centromeric cohesion in somatic cells [GIP2] PMID: 26124146
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center. Cytoplasm, cytoskeleton, spindle. Nucleus. Cytoplasm, cytoskeleton, phragmoplast. Nucleus envelope. Note=Reorganized from the nucleus to the prospindle and the preprophase band in late G2. After nuclear envelope breakdown, localized on spindle and phragmoplast microtubules (MTs) and on the reforming nuclear envelope of daughter cells. Present in mitotic microtubule arrays. In interphase cortical arrays, gamma-tubulin complexes are preferentially recruited to existing microtubules, from which new microtubules are efficiently nucleated.
Protein Families
MOZART1 family
Tissue Specificity
Mostly expressed in siliques and flowers, and, to a lower extent, in leaves, roots and seedlings, with highest levels in young tissues, meristematic cells, and the vasculature.
Database Links

KEGG: ath:AT1G73790

STRING: 3702.AT1G73790.1

UniGene: At.34954

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