Recombinant Arabidopsis thaliana Monogalactosyldiacylglycerol synthase 1, chloroplastic (MGD1)

Code CSB-YP529318DOA
MSDS
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Source Yeast
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Code CSB-EP529318DOA
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Source E.coli
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Code CSB-EP529318DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP529318DOA
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Source Baculovirus
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Code CSB-MP529318DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MGD1
Uniprot No.
Alternative Names
MGD1; MGDA; At4g31780; F28M20.30Monogalactosyldiacylglycerol synthase 1; chloroplastic; AtMGD1; EC 2.4.1.46; MGDG synthase type A
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
107-533
Target Protein Sequence
GVGL SSDENGIREN GTGGVLGEEG LPLNGVEADR PKKVLILMSD TGGGHRASAE AIRAAFNQEF GDEYQVFITD LWTDHTPWPF NQLPRSYNFL VKHGTLWKMT YYGTSPRIVH QSNFAATSTF IAREIAQGLM KYQPDIIISV HPLMQHVPLR VLRSKGLLKK IVFTTVITDL STCHPTWFHK LVTRCYCPST EVAKRAQKAG LETSQIKVYG LPVRPSFVKP VRPKVELRRE LGMDENLPAV LLMGGGEGMG PIEATARALA DALYDKNLGE AVGQVLIICG RNKKLQSKLS SLDWKIPVQV KGFITKMEEC MGACDCIITK AGPGTIAEAM IRGLPIILNG YIAGQEAGNV PYVVENGCGK FSKSPKEISK IVADWFGPAS KELEIMSQNA LRLAKPEAVF KIVHDMHELV RKKNSLPQLS CTA
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the synthesis of the major structural component of photosynthetic membranes. Required for proper thylakoid membrane biogenesis. Does not discriminate between prokaryotic (18:1/16:0) or eukaryotic (18:2/18:2) 1,2-diacylglycerol species, but operates with some preference for the prokaryotic one. Is responsible for most galactolipid synthesis in chloroplasts. Required for the formation of thylakoid membranes and functional photosynthetic electron transport during cotyledons greening in young seedlings. May link galactolipid synthesis with the coordinated transcriptional regulation of chloroplasts and other organelles during cotyledon greening.
Gene References into Functions
  1. Data show that Monogalactosyldiacylglycerol (MGDG) synthase 1 (MGD1) has a long and flexible region (approximately 50 amino acids) that is required for diacylglycerol (DAG) binding. PMID: 26935252
  2. MGD1 function may link galactolipid synthesis with the coordinated transcriptional regulation of chloroplasts and other organelles during cotyledon greening. PMID: 25253888
  3. A role of these lipids on the localization of MGD1 in specific domains. PMID: 24692595
Subcellular Location
Plastid, chloroplast inner membrane.
Protein Families
Glycosyltransferase 28 family
Tissue Specificity
Expressed in roots, stems, leaves, flowers, siliques and seeds.
Database Links

KEGG: ath:AT4G31780

STRING: 3702.AT4G31780.2

UniGene: At.1647

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